Immunocytochemical Differentiation of Lymphocytic Effusions

  • P. Spieler
  • U. Schmid
Conference paper


The cytological diagnosis of malignant lymphoma in serous effusions can be difficult because reactive lymphocytes may be morphologically indistinguishable from malignant cells in lymphocytic and other low-grade non-Hodgkin’s lymphomas (NHL). Possible causes of lymphocyte-rich effusions are: viral pleuropneumonia, tuberculous pleurisy, infarct (lung), trauma (repeated paracentesis), irradiation, malignant lymphoma, carcinosis of serous membranes. A few recent studies have shown that some of the diagnostic problems can be clarified with B and T cell enumeration using immunocytochemical techniques (Ghosh et al.1985; Martin et. al.1984). The immunocytochemical labelling technique can be applied to routinely performed cytological smears. The laboratory procedure is simple, the material may be stored for long periods, and the labelled cells exhibit well-preserved morphological details.


Malignant Lymphoma Serous Membrane Cytological Smear Immunological Result Serous Effusion 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Domagala W, Emeson EE, Koss LG (1981) T and B lymphocyte enumeration in the diagnosis of lymphocyte-rich pleural fluids. Acta Cytol (Baltimore) 25:108–110Google Scholar
  2. Ghosh AK, Spriggs AI, Mason DY (1985) Immunocytochemical staining of T and B lymphocytes in serous effusions. J Clin Pathol 38: 608–612PubMedCrossRefGoogle Scholar
  3. Krajewsky AS, Dewar AE, Ramage EF (1982) T and B lymphocyte markers in effusions of patients with non-Hodgkin’s lymphoma. J Clin Pathol 35: 1216–1219CrossRefGoogle Scholar
  4. Martin SE, Zhang HZ, Magyarosy E, Jaffe ES, Hsu SM, Chu EW (1984) Immunologic methods in cytology: definitive diagnosis of non-Hodgkin’s lymphomas using immunologic markers for T-and B-cells. Am J Clin Pathol 82: 666–673PubMedGoogle Scholar
  5. Spieler P, Kradolfer D, Schmid U (1986) Immunocytochemical characterization of lymphocytes in benign and malignant lymphocyte-rich serous effusions. Virchows Arch [A] 409: 211–221CrossRefGoogle Scholar
  6. Walts AE, Said JW (1983) Specific tumor markers in diagnostic cytology. Acta Cytol (Baltimore) 27: 408–416Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1988

Authors and Affiliations

  • P. Spieler
  • U. Schmid

There are no affiliations available

Personalised recommendations