Abstract
In situ hybridization has not up to now been seen as a tool for the pathology laboratory, but rather as a specialist technique applied by geneticists and some other molecular biologists. As the power of the technique to answer important biological questions becomes more appreciated, and as the technology advances and becomes both more sensitive and more adaptable, this is likely to change. In the future it is likely that it will have some quite routine applications.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Bartram CR, de Klein A, Hagemeijer A, van Agthoven T, Guerts van Kessel A, Bootsma D, Grosveld G, Ferguson-Smith MA, Davies T, Stone M, Heisterkamp N, Stephenson J, Groffen J (1983) Translocation of c-abl oncogene correlates with the presence of a Philadelphia chromosome in chronic myelocytic leukaemia. Nature 306: 277–280
Buckle VJ, Craig IW (1986) In situ hybridization. In: Davies KE (ed) Human genetics: a practical approach. IRL Press, Oxford, pp 85–100
Chandler MF, Yunis JJ (1978) A novel banding method for in situ hybridization. Cytogenet Cell Genet 22: 352–356
Davis MB, Malcolm S, Rabbitts TH (1984) Chromosome translocation can occur on either side of the c-myc oncogene in Burkitt’s lymphoma cells. Nature 308: 286–288
Donis-Keller H et al. (1987) Genetic linkage map of the human chromosomes. Cell 51: 319–337
Garson JA, van den Berghe JA, Kemshead JT (1987 a) Novel non isotopic in situ hybridization technique detects small (1 kb) unique sequences in routinely G-banded human chromosomes: fine mapping of N-myc and BNGF genes. Nucleic Acids Res 15: 4761–4770
Garson JA, van den Berghe JA, Kemshead JT (1987 b) High resolution in situ hybridization technique using biotinylated NMYC oncogene probe reveals periodic structure of HSRs in human neuroblastomas. Cytogenet Cell Genet 45:10–15
Human Gene Mapping 8 Helsinki Conference (1985) 8. Internat. Workshop on human Gene Mapping. Cytogenet Cell Genet 40: Nos. 1–4
Kirsch IR, Morton CC, Nakahara K, Leder P (1982) Human immunoglobulin heavy chain genes map to a region of translocation in malignant B lymphocytes. Science 216: 301–303
Langer PR, Waldrop AA, Ward DC (1981) Enzymatic synthesis of biotin-labelled polynucleotides: novel nucleic acid affinity probes. Proc Natl Acad Sci USA 78: 6633–6637
Malcolm S (1986) Specialist techniques in research and diagnostic clinical cytogenetics. In: Rooney D, Czepulkowski B (eds) Human cytogenetics: a practical approach. IRL Press, Oxford, pp 197–226
Malcolm S, Barton P, Murphy C, Ferguson-Smith MA (1981) Chromosomal localisation of a single copy gene by in situ hybridization. Ann Hum Genet 45:135–141
Penketh R, Monk M, van den Berghe JA, Delhanty J, Handyside A, Malcolm S, Middleton-Price HR, Tavares M, Pembrey M, Winston R (in press) Prospects for diagnosis in pre-implantation embryos. J Med Genet (in press)
Schwab M, Alitalo K, Klempnauer K-H, Varmus HE, Bishop JM, Gilbert F, Brodeur G, Goldstein M, Trent J (1983) Amplified DNA with limited homology to myc cellular oncogene is shared by human neuroblastoma cell lines and neuroblastoma tumours. Nature 305: 245–248
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1988 Springer-Verlag Berlin Heidelberg
About this paper
Cite this paper
Malcolm, S., van den Berghe, J. (1988). In Situ Hybridization: Its Use as a Tool in Pathology Laboratories. In: Goerttler, K., Feichter, G.E., Witte, S. (eds) New Frontiers in Cytology. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-73596-7_3
Download citation
DOI: https://doi.org/10.1007/978-3-642-73596-7_3
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-73598-1
Online ISBN: 978-3-642-73596-7
eBook Packages: Springer Book Archive