Skip to main content
SpringerLink
Log in

DNA Flow Cytometry in Diagnostic Cytopathology: Methodologic Considerations and Current State of Clinical Applications

  • Conference paper
New Frontiers in Cytology

  • 97 Accesses

Abstract

The recent progress of flow cytometry was made possible by technical improvement of the flow cytometers, by developments in computer techniques, with better possibilities of data storage and evaluation, and by increased experience with the methods of staining and preparation.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 84.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Bauer KD, Merkel DE, Winter JN, Marder RJ, Hauck WW, Wallemark CB, Williams TJ, Variakojis D (1986) Prognostic implications of ploidy and proliferative activity in diffuse large cell lymphomas. Cancer Res 46: 3173–3178

    PubMed  CAS  Google Scholar 

  • Bauer KD, Lincoln ST, Vera-Roman JM, Wallemark CB, Chmiel JS, Madurski ML, Murad T, Scarpelli DG (1987) Prognostic implications of proliferative activity and DNA aneuploidy in colonic adenocarcinomas. Lab Invest 57: 329–335

    PubMed  CAS  Google Scholar 

  • Berndt R, Feichter G (1987) DNS-Durchflußzytophotometrie, histologisches Typing und Grading bei Weichteilsarkomen. Verh Dtsch Ges Path 71: 350

    Google Scholar 

  • Bunn PA, Carney DN, Gazdar AF, Whang-Peng J, Matthews MJ (1983) Diagnostic and biological implications of flow cytometric DNA content analysis in lung cancer. Cancer Res 43. 5026–5032

    PubMed  CAS  Google Scholar 

  • Coon JS, Landay AL, Weinstein RS (1987) Advances in flow cytometry for diagnostic pathology. Lab Invest 57: 453–479

    PubMed  CAS  Google Scholar 

  • Cornelisse CJ, van de Velde CJH, Caspers RJC, Moolenaar AJ, Hermans J (1987) DNA ploidy and survival in breast cancer. Cytometry 8: 225–234

    Article  PubMed  CAS  Google Scholar 

  • Czech W (1987) Vergleich von Labeling-Index nach 3H-Thymidin-Markierung und Zellzyklusanalyse durch DNA-Impulszytophotometrie zur Quantifizierungg DNA-synthetisierender Zellen. Thesis, University of Heidelberg

    Google Scholar 

  • Emdin StO, Stenling R, Roos G (1987) Prognostic value of DNA content in colorectal carcinoma. A flow cytometric study with some methodologic aspects. Cancer 60: 1282–1287

    Article  PubMed  CAS  Google Scholar 

  • Feichter GE, Höffken H, Heep J, Haag D, Heberling D, Brandt H, Rummel H, Goerttler K (1982) DNA-flow-cytometric measurements on the normal, atrophic, hyperplastic and neoplastic human endometrium. Virchows Arch Pathol Anat 398: 53–65

    Article  CAS  Google Scholar 

  • Feichter GE, Kühn W, Czernobilsky B, Müller A, Heep J, Abel U, Haag D, Kaufmann M, Rummel HH, Kubli F, Goerttler K (1985) DNA flow cytometry of ovarian tumors with correlation to histopathology. Int J Gynecol Pathol 4: 336–345

    Article  PubMed  CAS  Google Scholar 

  • Feichter GE, Maier H, Adler D, Born IA, Abel U, Haag D, Goerttler K (1987) S-phase fractions and DNA-ploidy of oropharyngeal squamous epithelium carcinomas. Acta Otolaryngol 104: 377–384

    Article  PubMed  CAS  Google Scholar 

  • Feichter GE, Müller A, Kaufmann M, Haag D, Born IA, Abel U, Klinga K, Kubh F, Goerttler K (1988) Correlation of DNA flow cytometric results and other prognostic factors in breast cancer. Int J Cancer (in press)

    Google Scholar 

  • Frankfurt OS, Chin JL, Englander LS, Greco WR, Pontes JE, Rustum YM (1985) Relationship between DNA ploidy, glandular differentiation, and tumor spread in human prostate cancer. Cancer Res 45:1418–1423

    PubMed  CAS  Google Scholar 

  • Friedlander ML, Hedley DW, Taylor IW, Russel P, Coated AS, Tattersall (1984) Influence of cellular DNA content on survival in advanced ovarian cancer. Cancer Res 44: 397–400

    PubMed  CAS  Google Scholar 

  • Geisinger KR, Kute TE, Marshall RB, Homesley HD, Morgan TM (1986) Analysis of the relationships of the ploidy and cell cycle kinetics to differentiation and the female sex steroid receptors in adenocarcinoma of the endometrium. Am J Clin Pathol 85: 397–400

    Google Scholar 

  • Giaretti W, Sciallero S, Bruno S, Geido E, Aste H, DFi Vinci A (1988) DNA flow cytometry of endoscopically examined colorectal adenomas and adenocarcinomas. Cytometry 9: 238–244

    Article  PubMed  CAS  Google Scholar 

  • Haag D, Feichter GE, Goerttler K, Kaufmann M (1987) Influence of systematic errors on the evaluation of the S-phase portions from DNA distributions of solid tumors as shown for 328 breast carcinomas. Cytometry 8: 377–385

    Article  PubMed  CAS  Google Scholar 

  • Hedley DW, Rugg CA, Gelber RD (1987) Association of DNA index and S-phase fraction with prognosis of N+ early breast cancer. Cancer 47: 4729–4735

    CAS  Google Scholar 

  • Horsfall DJ, Tilley WD, Orell SR, Marshall VR, Cant ELMcK (1986) Relationship between ploidy and steroid hormone receptors in primary invasive breast cancer. Br J Cancer 53: 23–28

    Article  PubMed  CAS  Google Scholar 

  • Iversen OE, Skaarland E (1987) Ploidy assessment of benign and malignant ovarian tumors by flow cytometry. Cancer 60: 82–87

    Article  PubMed  CAS  Google Scholar 

  • Johnson TS, Williamson KD, Cramer MM, Peters U (1985) Flow cytometric analysis of head and neck carcinoma DNA Index and S-fraction from paraffin-embedded sections: comparison with malignancy grading. Cytometry 6: 461–470

    Article  PubMed  CAS  Google Scholar 

  • Kallioniemi OP, Blanco G, Alavaikko M, Hietanen T, Mattile J, Lauslahti K, Koivula T (1987) Tumor DNA ploidy as an independent prognostic factor in breast cancer. Br J Cancer 56: 637–642

    Article  PubMed  CAS  Google Scholar 

  • Kaufmann M, Feichter GE, Nhila A, Klinga K, Abel U (1988) Flow-zytophotometnsche Parameter, Hormonrezeptoren und axillärer Lymphknotenstatus als Prognosefaktoren beim Mamma-karzinom. Geburtsh u Frauenheilk (in press)

    Google Scholar 

  • Kreicbergs A, Tribukait B, Willems J, Bauer HCF (1987) DNA flow analysis of soft tissue tumors. Cancer 59:128–133

    Article  PubMed  CAS  Google Scholar 

  • Kühn W, Feichter GE, Hanke J, Rummel HH, Kaufmann M, Schmid H (1987) Klinischer Verlauf des Ovarialkarzinoms in Abhängigkeit von morphologischen Prognosefaktoren und zellkinetischen Parametern. Geburtsh u Frauenheilk 47: 446–451

    Article  Google Scholar 

  • Leidinger J (1987) Beziehungen zwischen DNS-durchflußzytophotometrisch ermittelten S-Phasen, mammographisch bestimmter Tumorverdoppelungszeit und Ãœberlebenszeit beim Mammakarzinom. Thesis, University of Heidelberg

    Google Scholar 

  • Lenner P, Johansson H, Lindh J, Dige U (1987) Non-Hodgkin-Lymphoma. Multivariate analysis of prognostic factors including fraction of S-phase cells. Acta Oncol 26:179–183

    Article  PubMed  CAS  Google Scholar 

  • Lindahl B, Alm P, Fernö M, Killander D, Langström E, Norgren A, Trope C (1987) Prognostic value of flow cytometrical DNA measurements in stage I-II endometrial carcinoma. Anticancer Res 7: 791–798

    PubMed  CAS  Google Scholar 

  • Lundberg S, Carstensen J, Rundquist I (1987) DNA flow cytometry and histopathological grading of paraffin-embedded prostate biopsy specimens in a survival study. Cancer Res 47: 1973–1977

    PubMed  CAS  Google Scholar 

  • McDivitt RW, Stone KR, Craig RB, Palmer JO, Meyer JS, Bauer WC (1986) A proposed classification of breast cancer on kinetic information derived from a comparison of risk factors in 168 primary operable breast cancers. Cancer 57: 269–276

    Article  PubMed  CAS  Google Scholar 

  • Merkel DE, Dressier LG, McGuire WL (1987) Flow cytometry, cellular DNA content, and prognosis in human malignancy. J Clin Oncol 5:1690–1703

    PubMed  CAS  Google Scholar 

  • Moran RE, Melamed MR (1984) Flow cytometric analysis of human lung cancer. Correlation with histologic type and stage. Anal Quant Cytol 6: 99–104

    PubMed  CAS  Google Scholar 

  • Murphy WM (1987) DNA flow cytometry in diagnostic pathology of the urinary tract. Human Pathol 18: 317–319

    Article  CAS  Google Scholar 

  • Oud PS, Reubsaet-Veldhuizen JAM, Beck HLM, Pahlplatz MMM, Hesselmanns GHFM, Hermkens HG, Tas J, James J, Vooijs GP (1986) DNA and nuclear protein measurement in columnar epithelial cells of human endometrium. Cytometry 7: 325–330

    Article  PubMed  CAS  Google Scholar 

  • Owainati AAR, Robins RA, Hinton C, Ellis IO, Dowle CS, Ferry B, Elston CW, Blarney RE, Baldwin RW (1987) Tumour aneuploidy, prognostic parameters and survival in primary breast cancer. Br J Cancer 55: 449–454

    Article  PubMed  CAS  Google Scholar 

  • Quirke P, Fozard JBJ, Dixon MF, Dyson JED, Giles GR, Bird CC (1986) DNA aneuploidy in colorectal adenomas. Br J Cancer 53: 477–481

    Article  PubMed  CAS  Google Scholar 

  • Ramaekers CS, Beck HLM, Feitz WFJ, Oud PS, Debruyne FMJ, Vooijs GP, Herman ChJ (1986) Application of antibodies to intermediate filament proteins as tissuespecific probes in flow cytometric analysis of complex tumors. Anal Quant Cytol 8: 271–280

    CAS  Google Scholar 

  • Rodenburg CJ, Cornelisse CJ, Heintz PAM, Hermans J, Fleuren GJ (1987) Tumor ploidy as a major prognostic factor in advanced ovarian cancer. Cancer 59: 317–323

    Article  PubMed  CAS  Google Scholar 

  • Rutgers DH, Wils IS, Schaap AH, von Lindert ACM (1987) DNA flow cytometry, histological grade, stage, and age as prognostic factors in human epithelial ovarian carcinomas. Pathol Res Pract 182: 207–213

    Article  PubMed  CAS  Google Scholar 

  • Scott NA, Wieand HS, Moertel ChG, Cha StS, Beart RW, Lieber MM (1987) Colorectal cancer: Duke’s stage, tumor site, preoperative plasma CEA level, and patient prognosis related to DNA ploidy pattern. Arch Surg 122:1375–1379

    PubMed  CAS  Google Scholar 

  • Strang P, Eklund G, Stendahl U, Frankendahl B (1987) S-phase rate as a predictor of early recurrences in carcinoma of the uterine cervix. Anticancer Res 7: 807–810

    PubMed  CAS  Google Scholar 

  • Tirindelli-Danesi D, Teodori L, Mauro F, Modini C, Botti C, Ciocconetti F, Stipa S (1987) Prognostic significance of flow cytometry in lung cancer. A 5-year study. Cancer 60: 844–851

    Article  PubMed  CAS  Google Scholar 

  • Tribukait B (1987) Flow cytometry in assessing the clinical aggressiveness of genito-urinary neoplasms. World J Urol 5: 108–122

    Article  Google Scholar 

  • Tytor M, Franzèn G, Olofsson J, Brunk U, Nordenskjöld B (1987) DNA content, malignancy grading and prognosis in Tl and T2 oral cavity carcinomas. Br J Cancer 56: 647–662

    Article  PubMed  CAS  Google Scholar 

  • Volm M, Brüggemann A, Günther M, Kleine W, Pfleiderer A, Vogt-Schaden M (1985 a) Prognostic relevance of ploidy, proliferation, and resistance-predictive tests in ovarian carcinoma. Cancer Res 45: 5180–5185

    PubMed  CAS  Google Scholar 

  • Volm M, Drings P, Mattern J, Sonka J, Vogt-Moykopf I, Wayss K (1985 b) Prognostic significance of DNA patterns and resistance-predictive tests in non-small cell lung carcinoma. Cancer 56: 1396–1403

    Article  PubMed  CAS  Google Scholar 

Download references

Authors
  1. G. E. Feichter
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 280, D-6900, Heidelberg, Federal Republic of Germany

    Klaus Goerttler

  2. Institut für Vergleichende und Experimentelle Pathologie der Universität, Im Neuenheimer Feld 220-221, D-6900, Heidelberg, Federal Republic of Germany

    Georg E. Feichter

  3. Abteilung Innere Medizin, Diakonissenhospital, D-7500, Karlsruhe, Federal Republic of Germany

    Siegfried Witte

Rights and permissions

Reprints and permissions

Copyright information

© 1988 Springer-Verlag Berlin Heidelberg

About this paper

Cite this paper

Feichter, G.E. (1988). DNA Flow Cytometry in Diagnostic Cytopathology: Methodologic Considerations and Current State of Clinical Applications. In: Goerttler, K., Feichter, G.E., Witte, S. (eds) New Frontiers in Cytology. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-73596-7_16

Download citation

  • DOI: https://doi.org/10.1007/978-3-642-73596-7_16

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-73598-1

  • Online ISBN: 978-3-642-73596-7

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation