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A Glycoprotein Surface Layer Covers the Pseudomurein Sacculus of the Extreme Thermophile Methanothermus fervidus

  • E. Nußer
  • E. Hartmann
  • H. Allmeier
  • H. König
  • G. Paul
  • K. O. Stetter

Abstract

With a temperature maximum of 97°C, Methanothermus is the most thermophilic methanogenic genus. Only members of this archaebacterial genus possess a double-layered cell envelope composed of a pseudomurein sacculus and an S-layer (Fig.1a; Stetter et al. 1981). Each layer could be specifically removed: the S-layer with detergent or pronase (Stetter et al. 1981) and the pseudomurein sacculi of freshly harvested cells with a pseudomurein endopeptidase from Methanobacterium wolfei (Fig.1d). The cells are bipolar polytrichous flagellated. Up to 10 flagella with a length of 8–10 μm and a thickness of 13–15 nm could be found at each pole. The channels at the two poles of the pseudomurein sacculi are probably the passage holes of the flagella, since the diameter corresponds with the thickness of the flagella (Fig.1a,b). The thickness and the rigidity of the cell envelopes varied with the culture conditions. The cell envelope layers became thicker and more rigid in autotrophically than in heterotrophically grown cells. The thickness of the pseudomurein and the S-layer increased from 14 nm and 10 nm to 22 nm and 16 nm, respectively.

Keywords

Cell Envelope Extreme Thermophile Alditol Acetate Derivative Passage Hole Thin Longitudinal Section 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

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  3. Wieland F, Lechner J, Sumper M (1982) The cell wall glycoprotein of halobacteria: Structural, functional and biosynthetic aspects. Zentralbl Bakteriol Hyg I Abt Orig C3:161–170Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1988

Authors and Affiliations

  • E. Nußer
    • 1
  • E. Hartmann
    • 1
  • H. Allmeier
    • 1
  • H. König
    • 1
  • G. Paul
    • 2
  • K. O. Stetter
    • 1
  1. 1.Lehrstuhl für MikrobiologieUniversität RegensburgRegensburgGermany
  2. 2.Lehrstuhl für Biochemie IUniversität RegensburgRegensburgGermany

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