Abstract
Forage legumes are universally considered to have higher feeding value than non-legumes and this, for the most part, is true. One of the main reasons why they are superior is the fact that in general they contain a higher percentage of protein and minerals than non-legumes. As a matter of fact leaves and stems of forage legumes are higher particularly in protein than other plants when they are harvested at a similar stage of maturity. Legumes not only have a higher percentage of protein, they also have high quality protein. This is of prime importance and helps greatly in obtaining high nutritive value in feeds for animals (Wheeler 1950). The quality of the protein of legumes is such to make them especially valuable as feed to supplement cereal grains lacking the proper protein for a balanced livestock feed. Besides being of special value for feed, legumes are superior for soil improvement, due to the large amount of nitrogen they are able to supply to the soil for the use of subsequent crops. In the legumes, taking nitrogen from the air is accomplished through symbiotic bacteria (Rhizobia) that develop in nodules on the roots of the legumes. These bacteria take nitrogen direct from the air as they grow and multiply in the nodule. The nitrogen in turn becomes available to the legume plant and aids its nourishment and growth. It is this symbiotic association of legumes and Rhizobia that gives legumes a distinct advantage over non-legume plants. In this chapter, in vitro culture studies on three forage legumes, i.e. Lotus corniculatus, Coronilla varia and Onobrychis viciifolia are detailed.
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Ahuja PS, Hadiuzzaman S, Davey MR, Cocking EC (1983a) Prolific plant regeneration from protoplast-derived tissues of Lotus corniculatus L. (Birdsfoot Trefoil). Plant Cell Rep 2:101–104
Ahuja PS, Lu DY, Cocking EC, Davey MR (1983b) An assessment of the cultural capabilities of Trifolium repens L. (white clover) and Onobrychis viciifolia Scop. (Sainfoin) mesophyll protoplasts. Plant Cell Rep 2:269–272
Anderson EJ (1959) Pollination of crownvetch. Glean Bee Cult 87:590–593
Arcioni S, Mariotti D (1983) Tissue culture and plant regeneration in Onobrychis viciifolia Scop. Z Pflanzenzucht 90:192–197
Arcioni S, Davey MR, Dos Santos AVP, Cocking EC (1982) Somatic embryogenesis in tissues from mesophyll and cell suspension protoplasts ofMedicago coerulea and M. glutinosa. Z Pflanzen- physiol 106:105–110
Arcioni S, Mariotti D, Damiani F, Pezzotti M (1986) Somaclonal variation in Lotus corniculatus L. In: Horn W, Jensen CJ, Odenbach W, Schieder O: Genetic manipulation in plant breeding, pp. 581–584. Proc Int Symp Eucarpia, Berlin (West), Germany, Sept 1985, Walter de Gruyter, Berlin New York
Auld DL, Ditterline RL, Mathre DE (1977) Screening sainfoin for resistance to root and crown rotcaused by Fusarium solani (Mart.). App Wr Crop Sei 17:69–73
Bocsa I (1980) Amélioration du rendement en matière sèche chez la Luzerne. In: Bocsa I (ed) Proc Eucarpia Fodoler Crop Sec, GATE Agric Res Inst, Kompolt, Hungary, pp 19–28
Cocking EC (1981) Opportunities from the use of protoplasts. Philos Trans R Soc London Ser B 292:557–568
D’Amato F (1977) Cytogenetics of differentiation in tissue and cell cultures. In: Reinert J, Bajaj YPS (eds) Applied and fundamental aspects of plant cell, tissue, and organ culture. Springer, Berlin Heidelberg New York, pp 343–357
Damiani F, Mariotti D, Pezzotti M, Arcioni S (1985) Variation among plants regenerated from tissueculture of Lotus corniculatus L. Z Pflanzenzucht 94:332–339
Davey MR (1983) Recent development in the culture and regeneration of plant protoplasts. In: Potrykus I, Harms CT, Hinnen A, Hütter R, King PJ, Shillito RD (eds) Protoplast 1983. 6th Int Protoplast Symp, Basel, August 1983. Birkhäuser, Basel Boston Stuttgart, pp 19–30
Dos Santos AVP, Outka DE, Cocking EC, Davey MR (1980) Organogenesis and somatic embryogenesis in tissue derived from leaf protoplasts and leaf expiants of Medicago sativa. Z Pflanzenphysiol 99:261–270
Duke JA (1981) Legumes species. In: Duke JA (ed) Handbook of legumes of world economic importance. Plenum, New York London, pp 125–129
Frearson EM, Power JB, Cocking EC (1973) The isolation, culture and regeneration of Petunia leaf protoplasts. Dev Biol 33:130–137
Gamborg OL, Miller RA, Ojina K (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res 50:151–158
Grant WF, Sidhu BS (1967) Basic chromosome number, cyanogenetic glucoside variation, and geographic distribution of Lotus species. Can J Bot 47:639–647
Gresshoff PM (1980) In vitro culture of white clover: callus, suspension, protoplast culture and plant regeneration. Bot Gaz 141:157–164
Gresshoff PM, Doy CH (1972a) Haploid Arabidopsis thaliana callus and plants from anther culture. Austr J Biol Sei 25:259–269
Gresshoff PM, Doy CH (1972b) Development and differentiation of haploidLycopersium esculen-tum. Planta 107:161–170
Gustine DL (1979) Aliphatic nitro-compounds in crownvetch: review. Crop Sei 19:197–203
Halperin W, Wetherell DF (1965) Ammonium requirement for embryogenesis in vitro. Nature (London) 205:519–520
Henson PR, Schoth HA (1962) The trefoils-adaptation and culture. USDA Agric Handb 223:1–16
Kao KN (1977) Chromosomal behaviour in somatic hybrids of soybean Nicotiana glauca. Mol Gen Genet 150:225–230
Kao KN, Michayluk MR (1975) Nutritional requirements for growth of Vicia hajastana cells and protoplasts at a very low population density in liquid medium. Planta 126:105–110
Kao KN, Miehayluk MR (1980) Plant regeneration from mesophyll protoplasts of alfalfa. Z Pflanzenphysiol 96:135–141
Kenson PR (1963) Crownvetch — A soil conserving legumes and a potential pasture and hay plant. USDA Agric Res Serv 34–53:1–9
Keyes GJ, Bingham ET (1979) Heterosis and ploidy effects on the growth of alfalfa callus. Crop Sci 19:473–476
Keyes GJ, Collins GB, Taylor NL (1980) Genetic variation in tissue culture of red clover. Theor Appl Genet 58:265–271
Larkin PJ, Scowcroft WR (1981) Somaclonal variation — A novel source of variability from cell cultures for plant improvement. Theor Appl Genet 60:197–214
Larkin PJ, Brettell R, Ryan S, Scowcroft W (1983) Protoplasts and variation from culture. In: Potrykus I, Harms CT, Hinnen A, Hutter R, King PJ, Shillito RD (eds) Protoplast 1983. 6th Int Protoplast Symp, Basel, August 1983, Birkhauser, Basel Boston Stuttgart, pp 51–56
Linsmaier EM, Skoog F (1965) Organic growth factor requirements of tobacco tissue cultures. Physiol Plant 18:100–127
Mariotti D, Arcioni S (1983) Callus culture ofCoronilla varia L. (crownvetch): plant regeneration through somatic embryogenesis. Plant Cell Tissue Org Cult 2:103–110
Mariotti D, Pezzotti M, Falistocco E, Arcioni S (1984) Plant regeneration from leaf-derived callus of Lotus corniculatus cv. Franco. Genet Agric 38:219–223
McCoy TJ, Bingham ET (1977) Regeneration of diploid alfalfa plants from cells grown in suspension culture. Plant Sci Lett 10:59–64
Miller CO (1963) Kinetin and kinetin-like compounds. In: Linskens HF, Tracey MV (eds) Moderne Methoden der Pflanzenanalyse, vol 6. Springer, Berlin Heidelberg New York, pp 192–202
Moyer BG, Gustine DL (1984) Regeneration ofCoronilla varia L. (crownvetch) plants from callus culture. Plant Cell Tissue Org Cult 3:143–148
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant 15:473–497
Niizeki M, Grant WF (1971) Callus, plantlet formation, and polyploidy from cultured anthers of Lotus and Nicotiana. Can J Bot 49:2041–2051
Orshinski BR, Tomes DT (1984) Comparison of plants derived from cuttings, node cultures, and ethyl methanesulfonate treated node cultures of birdsfoot trefoil (Lotus corniculatus). Can J Bot 62:1501–1504
Orshinski BR, Tomes DT (1985) Effect of long term culture and low temperature incubation on plant regeneration from a callus line of birdsfoot trefoil (Lotus corniculatus L.). J Plant Physiol 119:389–397
Orshinsky BR, Swanson EB, Tomes DT (1983) Enhanced shoot regeneration from homogenized callus cultures of birdsfoot trefoil (Lotus corniculatus L.). Plant Cell Tissue Org Cult 2:341–347
Pezzotti M, Arcioni S, Damiani F, Mariotti D (1985) Time-related behaviour of phenotypic variation in Lotus corniculatus regenerants under field conditions. Euphytica 34:619–623
Phillips RL (1968) Cyanogenesis inLotus species. Crop Sci 8:123–124
Prat D (1983) Genetic variability induced in Nicotiana sylvestris by protoplast culture. Theor Appl Genet 64:223–230
Reisch B (1982) Variability among plants from Ethionine resistant alfalfa tissue cultures. In: Fujiwara A (ed) Plant tissue culture 1982. Maruzen, Tokyo, pp 415–416
Sarno R (1981) Lupinella(Onobrychis viciifolia Scop.) (sin. O. sativa Lam.). In: Baldoni R, Giardini L (eds) Coltivazioni erbacee. Patron, Bologna, pp 891–893
Saunders JW, Bingham ET (1972) Production of alfalfa plants from callus tissue. Crop Sci 12:804–807
Seaney RR (1964) Cross and self seed set in birdsfoot trefoil plants selected for self-fertility. Crop Sci 4:440–441
Seaney RR, Henson PR (1970) Birdsfoot trefoil. Adv Agron 22:119–157
Schenk RU, Hildebrandt AC (1972) Medium and techniques for induction and growth of monocotyledonous and dicotyledonous cell cultures. Can J Bot 65:654–659
Shepard JF, Bidney D, Shahin E (1980) Potato protoplasts in crop improvement. Science 208:17–24
Swanson EB, Tomes DT (1980) Plant regeneration from cell cultures ofLotus corniculatus and the selection and characterization of 2,4-D tolerant cell lines. Can J Bot 58:1205–1209
Thomas E, Wernicke W (1978) Morphogenesis in herbaceous crop plants. In: Thorpe TA (ed) Frontiers of plant tissue culture. Proc 4th Int Congr Plant cell culture, Univ Calgary, pp 403–410
Thomas E, King PJ, Potrykus I (1979) Improvement of crop plants via single cell in vitro. An assessment. Z Planzenzucht 82:1–30
Thomas E, Bright WJ, Franklin J, Lancaster VA, Miflin BJ (1982) Variation amongst protoplast-derived potato plants (Solarium tuberosum cv. “Maris Bard”). Theor Appl Genet 62:65–68
Tomes DT (1978) A tissue culture procedure for propagation and maintenance of Lotus corniculatus genotypes. Can J Bot 57:137–140
Uchimiya H, Murashige T (1974) Evaluation of parameters in the isolation of viable protoplasts from cultured tobacco cells. Plant Physiol 54:936–944
Walker KA, Sato SG (1981) Morphogenesis in callus tissue ofMedicago sativa: the role of ammonium ions in somatic embryogenesis. Plant Cell Tissue Org Cult 1:109–121
Wernsman EA, Keim WF, Davis RL (1964) Meiotic behaviour in two Lotus species. Crop Sci 4:483–486
Wheeler WA (1950) Grasses and legumes as forage and pasture crops. In: Wheeler WA (ed) Forage and pasture crops. Van Nastrand, Princeton, NJ, pp 3–24
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Arcioni, S., Mariotti, D., Damiani, F., Pezzotti, M. (1988). Birdsfoot Trefoil (Lotus corniculatus L.), Crownvetch (Coronilla varia L.) and Sainfoin (Onobrychis viciifolia Scop.). In: Bajaj, Y.P.S. (eds) Crops II. Biotechnology in Agriculture and Forestry, vol 6. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-73520-2_31
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DOI: https://doi.org/10.1007/978-3-642-73520-2_31
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