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Suppression of Endotoxin-Induced Monokine Release by Human Serum Fractions

  • W. A. Flegel
  • H. Northoff
Conference paper

Abstract

Minute amounts of endotoxin (lipopolysaccharide, LPS) will induce monocytes concomitantly to release interleukin 1 (IL1), tumor necrosis factor (TNF), and interleukin 6 (IL6). This mechanism was shown (Lehmann et al. 1987; Freudenberg et al. 1986) to be central in mediating the in vivo toxicity of LPS. Therefore, the mediator release by human monocytes upon stimulation with LPS is a suitable and sensitive in vitro model for investigating the toxic effects of LPS. Inhibition of LPS to induce mediator release in this in vitro model by different means might have implications for diminished in vivo toxicity of endotoxin.

Keywords

Human Monocyte Mediator Release Lipoprotein Fraction Normal Human Serum Serum Fraction 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Fink PC, Lehr L, Urbaschek RM, Kozak J (1981) Limulus amebocyte lysate test for endotoxemia. Klin Wochenschr 59: 213–218PubMedCrossRefGoogle Scholar
  2. Freudenberg MA, Bøg-Hansen TC, Back U, Galanos C (1980) Interaction of lipopolysaccharides with plasma high-density lipoprotein in rats. Infect Immun 28: 373–380PubMedGoogle Scholar
  3. Freudenberg MA, Freudenberg N, Galanos C (1982) Time course of cellular distribution of endotoxin in liver, lungs and kidneys of rats. Br J Exp Pathol 63: 56–65PubMedGoogle Scholar
  4. Freudenberg MA, Keppler D, Galanos C (1986) Requirement for lipopolysaccharide-responsive macrophages in galactosamine-induced sensitization to endotoxin. Infect Immun 51: 891PubMedGoogle Scholar
  5. Lehmann V, Freudenberg MA, Galanos C (1987) Lethal toxicity of lipopolysaccharide and tumor necrosis factor in normal and d-galactosamine-treated mice. J Exp Med 165: 657–663PubMedCrossRefGoogle Scholar
  6. Munford RS, Andersen JM, Dietschy JM (1981) Sites of tissue binding and uptake in vivo of bacterial lipopolysaccharide-high density lipoprotein complexes. J Clin Invest 68: 1503–1513PubMedCrossRefGoogle Scholar
  7. Northoff H, Kabelitz D, Galanos C (1986) Interleukin 1 production for detection of bacterial polysaccharide in fetal calf sera and other solutions. Immunol Today 7: 126–127CrossRefGoogle Scholar
  8. Northoff H, Glück D, Wölpl A, Kubanek B, Galanos C (1987) Lipopolysaccharide-induced elaboration of interleukin 1 by human monocytes: use for detection of lipopolysaccharide in serum and the influence of serum-lipopolysaccharide interactions. Rev Infect Dis 9: S599 - S601PubMedCrossRefGoogle Scholar
  9. Obayashi T (1984) Addition of perchloric acid to blood samples for colorimetric limulus test using chromogenic substrate: comparison with conventional procedures and clinical applications. J Lab Clin Med 104: 321–330PubMedGoogle Scholar
  10. Ulevitch RJ, Johnston AR, Weinstein DB (1979) New function for high density lipoproteins. J Clin Invest 64: 1516–1524PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag, Berlin Heidelberg 1989

Authors and Affiliations

  • W. A. Flegel
  • H. Northoff

There are no affiliations available

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