Suppression of Endotoxin-Induced Monokine Release by Human Serum Fractions
Minute amounts of endotoxin (lipopolysaccharide, LPS) will induce monocytes concomitantly to release interleukin 1 (IL1), tumor necrosis factor (TNF), and interleukin 6 (IL6). This mechanism was shown (Lehmann et al. 1987; Freudenberg et al. 1986) to be central in mediating the in vivo toxicity of LPS. Therefore, the mediator release by human monocytes upon stimulation with LPS is a suitable and sensitive in vitro model for investigating the toxic effects of LPS. Inhibition of LPS to induce mediator release in this in vitro model by different means might have implications for diminished in vivo toxicity of endotoxin.
KeywordsHuman Monocyte Mediator Release Lipoprotein Fraction Normal Human Serum Serum Fraction
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- Northoff H, Glück D, Wölpl A, Kubanek B, Galanos C (1987) Lipopolysaccharide-induced elaboration of interleukin 1 by human monocytes: use for detection of lipopolysaccharide in serum and the influence of serum-lipopolysaccharide interactions. Rev Infect Dis 9: S599 - S601PubMedCrossRefGoogle Scholar