Detection of 4-Hydroxynonenal and Other Lipid Peroxidation Products in the Liver of Allyl Alcohol-Intoxicated Mice
Allyl alcohol has long been known to produce periportal necrosis of the liver in rats and mice (Miessner 1891; Piazza 1915). Also it is known that allyl alcohol is metabolized by the cytosolic enzyme alcohol dehydrogenase to acrolein (Rees and Tarlow 1967; Serafini-Cessi 1972). The latter is considered as one of the most important toxic metabolites responsible for the damage induced by allyl alcohol in liver and other tissues. Acrolein is in fact the most toxic member of the class of 2-alkenals (Beauchamp et al. 1985; Schauenstein et al. 1977), α-β unsaturated aldehydes which also include crotonaldehyde, pentenal, hexenal and so on. Acrolein is a powerful electrophile which reacts even spontaneously with nucleophiles such as sulphydryl groups (Esterbauer et al. 1975). The reaction is markedly accelerated by the activity of GSH-transferases. Cellular GSH is primarily involved in the reaction and the result is a dramatic loss of GSH stores (Hanson and Anders 1978; Zitting and Heinonen 1980; Dawson et al. 1984; Ohno et al. 1985; Jaeschke et al. 1987). The covalent binding of allyl alcohol metabolites to liver cells has been demonstrated with various techniques (Reid 1972).
KeywordsLipid Peroxidation Lipid Peroxidation Product Allyl Alcohol Malonic Dialdehyde Intoxicated Animal
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