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Safety of Baculoviruses used as Biological Insecticides

  • Jürg Huber

Abstract

About 1100 viruses are known to infect insects, more than 60% of them being baculoviruses (1). The members of the virus family Baculoviridae are characterized by double stranded circular DNA, included in rod-shaped capsids which are formed mostly in the nucleus of the host cells. As a feature in common with some insect viruses from other virus families, the virions of most baculoviruses are contained within the matrix of proteinaceous particles, the so called occlusion bodies (OB), which provide protection against adverse physical and chemical factors in the environment. Based on the morphology of the OBs, baculoviruses are divided into 3 subgroups: (a) nuclear polyhedrosis viruses (NPV), where many virions are contained in an OB, (b) granulosis viruses (GV), where every virion has its own OB and (c) the small group of non-occluded baculoviruses, where no OB is formed (2). The natural way of infection is by ingestion of food contaminated with virus. In the gut of the susceptible host the matrix protein of the OB is dissolved and virus particles are relaesed. They enter the gut epithelial cells, where they multiply and subsequently quickly spread to several other tissues of the host. Baculoviruses do not produce toxins, but their massive multiplication in vital tissues eventually leads to the death of the insect, usually within 1 to 2 weeks after infection.

Keywords

Wide Host Range Nuclear Polyhedrosis Virus Virus Family Occlusion Body Narrow Host Range 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Martignoni, E.M. and Iwai, P.J. (1986): A catalog of viral diseases of insects, mites and ticks. Gen. Tech. Rep. PNW-195, USDA, Forest Service, 51 p.Google Scholar
  2. 2.
    Matthews, R.E.F. (1982): Classification and nomenclature of viruses. Intervirol. 12, 1–199.CrossRefGoogle Scholar
  3. 3.
    Payne, C.C. (1986): Insect pathogenic viruses as pest control agents. Fortschr. Zoolog. 32, 183–200.Google Scholar
  4. 4.
    Podgewaite, J.D. (1986): Effects of insect pathogens on the environment. Fortschr. Zoolog. 32, 279–287.Google Scholar
  5. 5.
    Jaques, R.P. (1974): Occurrence and accumulation of the granulosis virus of Pieris rapae in treated field plots. J. Invertebr. Pathol. 23, 351–359.PubMedCrossRefGoogle Scholar
  6. 6.
    Podgewaite, J.D., Stone Shields, K., Zerillo, R.T. and Bruen, R.B. (1979): Environmental persistence of the nucleopolyhedrosis virus of the gypsy moth Lymantria dispar. Environ. Entomol. 8, 528–536.Google Scholar
  7. 7.
    Jaques, R.P. (1977): Stability of entomophathogenic viruses. Misc. Publ. Entomol. Soc. Am. 10(3), 99–116.Google Scholar
  8. 8.
    Huber, J. (1986): Use of baculoviruses in pest management programs. In: The Biology of Baculoviruses. Vol. II. Practical Application for Insect Control. (Eds. Granados, R.R. and Federici, B.A.), pp. 181–202, CRC Press, Boca Raton.Google Scholar
  9. 9.
    Falcon, L.A. (1980): Economical and biological importance of baculoviruses as alternatives to chemical pesticides. Proc. Symp. “Safety Aspects of Baculoviruses as Biological Insecticides”. 13.-15. Nov. 1978, Jülich, 27–46.Google Scholar
  10. 10.
    Burges, H.D., Croizier, G. and Huber, J. (1980): A review of safety tests on baculoviruses. Entomophaga 25, 329–340.CrossRefGoogle Scholar
  11. 11.
    Gröner, A. (1986): Specificity and safety of baculoviruses. In: The Biology of Baculoviruses. Vol. I. Biological Properties and Molecular Biology (Eds. Granados, R.R. and Federici, B.A.), pp. 177–202, CRS Press, Boca Raton.Google Scholar
  12. 12.
    Doerfler, W. (1986): Expression of the Autographa californica nuclear polyhedrosis virus genome in insect cells: Homologous viral and heterologous vertebrate genes - The baculovirus vector system. Curr. Top. Microbiol. Immunol. 131, 51–68.PubMedCrossRefGoogle Scholar
  13. 13.
    Martingoni, M.E. and Iwai, P.I. (1986): Propagation of multicapsid nuclear polyhedrosis virus of Orgyia pseudotsugata in larvae of Trichoplusia ni. J. Invertebr. Pathol. 47, 32–41.CrossRefGoogle Scholar
  14. 14.
    Veber, I. (1964): Virulence of an insect virus increased by repeated passages. Entomophaga, Mem. hors. Ser. 2, 403–405.Google Scholar
  15. 15.
    Brassel, J. and Benz, G. (1979): Selection of a strain of the granulosis virus of the codling moth with improved resistence against artificial ultraviolet radiation and sunlight. J. Invertebr. Pathol. 33, 358–363.CrossRefGoogle Scholar
  16. 16.
    Wood, H.A., Hughes, P.R., Johnston, L.B. and Longridge, W.H.R. (1981): Increased virulence of Autographa californica nuclear polyhedrosis virus by mutagenesis. J. Invertebr. Pathol. 38, 236–241.CrossRefGoogle Scholar
  17. 17.
    Mc Clintock, J.T. and Reichelderfer, C.F. (1985): In vivo treatment of a nuclear polyhedrosis virus of Autographa californica (Lepidoptera: Noctuidae) with chemical mutagens: Determination of changes in virulence in four Lepidopteran hosts. Environ. Entomol. 14, 691–695.Google Scholar
  18. 18.
    Brusca, J., Summers, M., Couch, J. and Courtney, L. (1986): Autographa californica nuclear polyhedrosis virus efficiently enters but does not replicate in poikilothermic vertebrate cells. Intervirol. 26, 207–222.CrossRefGoogle Scholar
  19. 19.
    Betz, F.S. (1986): Registration of baculoviruses as pesticides. In: The Biology of Baculoviruses. Vol. II. Practical Application for Insect Control (Eds. Granados, R.R. and Federici, B.A.) pp. 203–222, CRC Press, Boca Raton.Google Scholar
  20. 20.
    Croizier, G. and Quiot, J.M. (1981): Obtention and analysis of two genetic recombinants of baculoviruses of Lepidoptera, Autographa californica and Galleria mellonella. Ann. Virol. 132, 3–18.Google Scholar
  21. 21.
    Tjia, S.T., Meyer zu Altenschildesche, G. and Doerfler, W. (1983): Autographa californica nuclear polyhedrosis virus (AcNPV) DNA does not persist in mass cultures of mammalian cells. Virol. 125, 107–117.CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1988

Authors and Affiliations

  • Jürg Huber
    • 1
  1. 1.Biologische Bundesanstalt für Land- und ForstwirtschaftInstitut für biologische SchädlingsbekämpfungDarmstadtGermany

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