Strategy for Monitoring Pea-Nodulating Rhizobia without in Vitro Gene Manipulation

  • Wolfgang Lotz
Conference paper


A strategy is described for monitoring pea-nodulating bacteria of Rhizobium leguminosarum B10 (Nod+, Fix+, HUP+) without prior in vitro gene manipulation. The rhizobia are released into the soil of a selected test plot (having a low level of resident Hup+ R.leguminosarum bacteria) via sowing of B10-inoculated pea seeds. Seeds of the original host of strain B10, Pisum sativum. var. Poneka, are used for the release experiment and for further nodulation assays. Rhizobia isolated from sampled root nodules of pea plants grown in the test plot will be screened for the following properties: pattern of antibiotic resistance, presence of hup-specific DNA, UV-inducible bactericidal agent, plasmid pattern.


Rhizobium leguminosarum Pisum sativum hup genes plasmids seed inoculation monitoring strategy 


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  1. 1.
    Chancen und Risiken der Gentechnologie, Enquete-Kom. d. 10. Dt. Bundestages (1987). (Ed. Deutscher Bundestag, Referat öffentlichkeitsarbeit), Bonn.Google Scholar
  2. 2.
    Tichy, H.V. (1986). Klonierung und Analyse von Aufnahmehydrogenase (hup) Genen aus Rhizobium leguminosarum B10. Universität Erlangen-Nürnberg, Dissertation.Google Scholar
  3. 3.
    Eisbrenner, G. and Evans, H.J. (1983). Ann. Rev. Plant Physiol. 34, 105–136.CrossRefGoogle Scholar
  4. 4.
    Fees, H., Tichy, H.V. and Lotz, W. (1985). In: Nitrogen Fixation Research Progress (Eds. Evans, H.J., Bottomley, P.J. and Newton, W.E), p. 412, Martinus Nijhoff Publishers, Dordrecht.Google Scholar
  5. 5.
    Tichy, H.V. and Lotz, W. (1985). FEMS Microbiol. Lett. 27, 107–109.CrossRefGoogle Scholar
  6. 6.
    Tichy, H.V. (1981). Charakterisierung der Plasmide neu isolierter Rhizobium leguminosarum Stamme. Universität Erlangen-Nürnberg, Diplomarbeit.Google Scholar
  7. 7.
    Tichy, H.V, and Lotz, W. (1981). FEMS Microbiol. Lett. 10, 203–207.CrossRefGoogle Scholar
  8. 8.
    Cantrell, M.A., Haugland, R.A. and Evans, H.J. (1983). Proc. Natl. Acad. Sci. USA 80, 181–185.PubMedCrossRefGoogle Scholar
  9. 9.
    Tichy, H.V., Schild, C., Ripke, H.M., Nelson, L.M., Fees, H. and Lotz, W. (1987). In: Molecular Genetics of Plant-Microbe Interactions (Ed. Verma, D.P.S. and Brisson, N.), pp. 279–281, Martinus Nijhoff Publishers, Dordrecht.CrossRefGoogle Scholar
  10. 10.
    Zuber, M., Harker, A.R., Sultana, M.A. and Evans H.J. (1986). Proc. Natl. Acad. Sci. USA 83, 7668–7672.PubMedCrossRefGoogle Scholar
  11. 11.
    Date, R.A. and Roughley, R.J. (1977). In: A treatise on Dinitrogen Fixation, IV. Agronomy and Ecology (Eds. Hardy R.W.F. and Gibson, A.H.), pp. 243–275, John Wiley &Sons, New York.Google Scholar
  12. 12.
    Pain, A.N. (1979). J. Appl. Bacteriol. 47, 53–64.CrossRefGoogle Scholar
  13. 13.
    Lewis, D.M., Bromfield, E.S.P. and Barran, L.R. (1987). Can. J. Microbiol. 33, 343–345.CrossRefGoogle Scholar
  14. 14.
    Grunstein, M. and Hogness, D.S. (1975). Proc. Natl. Acad. Sci. USA 72, 3961–3965.PubMedCrossRefGoogle Scholar
  15. 15.
    Hodgson, A.L.M. and Roberts, W.P. (1983). J. Gen. Microbiol. 129, 207–212.Google Scholar
  16. 16.
    Cooper, J.E., Bjourson, A.J. and Thompson, J.K. (1987). Appl. Environ. Microbiol. 53, 1705–1707.PubMedGoogle Scholar
  17. 17.
    Cunningham, S. D., Kapulnik, Y. and Phillips, D.A. (1986). Appl. Environ. Microbiol. 52, 1091–1095.PubMedGoogle Scholar
  18. 18.
    Wong T.Y., Graham, L., O’Hara, E. and Maier, R.J. (1986). Appl. Environ. Microbiol. 52, 1008–1013.PubMedGoogle Scholar
  19. 19.
    Juni, E. and Janik, A. (1969). J. Bacteriol. 98, 281–288.PubMedGoogle Scholar
  20. 20.
    Nelson, L.M., Grosskopf, E., Tichy, H.V. and Lotz, W. (1985). FEMS Microbiol. Lett. 30, 53–58.Google Scholar
  21. 21.
    Eberz, G., Hogrefe, C., Kortlüke, C., Kamienski, A. and Friedrich, B. (1986). J. Bacteriol. 168, 636–641.PubMedGoogle Scholar
  22. 22.
    Friedrich, B., Kortlüke, C., Hogrefe, C., Eberz, G., Silber, B. and Warrelmann, J. (1986). Biochimie 68, 133–145.PubMedCrossRefGoogle Scholar
  23. 23.
    Arp, D.J.; McCollum, L.C. and Seefeldt, L.C. (1985). J. Bacteriol. 163, 15–20.PubMedGoogle Scholar
  24. 24.
    Kokotek, W. (1985). Isolierung und Charakterisierung eines bakteriziden Agens von Rhizobium leguminosarum B10. Universität Erlangen-Nürnberg, Diplomarbeit.Google Scholar
  25. 25.
    Beringer, J.E. (1974). J. Gen. Microbiol. 84, 188–198.PubMedGoogle Scholar
  26. 26.
    Seifert, B.L., Tichy, H.V., Nelson, L.M., Cantrell, M.A., Haugland, R.A. and Lotz, W. (1984). In: Advances in Nitrogen Fixation Research (Eds. Veeger, C., Newton, W.E.), p. 721, Martinus Nijhoff Publishers, The Hague.Google Scholar
  27. 27.
    Leyva, A., Palacios, J.M. and Ruiz-ArgUeso, T.R. (1987). Appl. Environ. Microbiol. 53, 2539–2543.PubMedGoogle Scholar
  28. 28.
    Brockwell, J. (1977). In: A Treatise on Dinitrogen Fixation, IV. Agronomy and Ecology (Eds. Hardy, R.W.F. and Gibson, A.H.), pp. 277–309, John Wiley and Sons, New York.Google Scholar
  29. 29.
    Williams, P.M. (1984). In: Biological Nitrogen Fixation (Ed. M. Alexander), pp. 173–200, Plenum Press, New York and London.CrossRefGoogle Scholar
  30. 30.
    Stacey, G. (1985). In: Engineered Organisms in the Environment: Scientific Issues (Eds. Halvorson, H.O., Pramer, D. and Rogul, M.), pp.109–121, American Society for Microbiology, Washington.Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1988

Authors and Affiliations

  • Wolfgang Lotz
    • 1
  1. 1.Institut für Mikrobiologie und Biochemie, Lehrstuhl für MikrobiologieUniversität Erlangen-NürnbergErlangenGermany

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