Summary
A strategy is described for monitoring pea-nodulating bacteria of Rhizobium leguminosarum B10 (Nod+, Fix+, HUP+) without prior in vitro gene manipulation. The rhizobia are released into the soil of a selected test plot (having a low level of resident Hup+ R.leguminosarum bacteria) via sowing of B10-inoculated pea seeds. Seeds of the original host of strain B10, Pisum sativum. var. Poneka, are used for the release experiment and for further nodulation assays. Rhizobia isolated from sampled root nodules of pea plants grown in the test plot will be screened for the following properties: pattern of antibiotic resistance, presence of hup-specific DNA, UV-inducible bactericidal agent, plasmid pattern.
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© 1988 Springer-Verlag Berlin Heidelberg
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Lotz, W. (1988). Strategy for Monitoring Pea-Nodulating Rhizobia without in Vitro Gene Manipulation. In: Klingmüller, W. (eds) Risk Assessment for Deliberate Releases. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-73419-9_5
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DOI: https://doi.org/10.1007/978-3-642-73419-9_5
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