Mating of haploid yeast strains can be done in a number of ways, either by mixing the yeast strains on a rich medium and isolating the resulting zygotes by micromanipulation, or by some variant of the drop-overlay method. The latter is frequently used for mating auxotrophic strains having complementary requirements. For prototrophic parental strains, of course, hybrids can only be isolated by micromanipulation of the zygotes from the mating mixture.
KeywordsGlycerol fIlters Agar Lactate Bromide
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