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Mating

  • John F. T. Spencer
  • Dorothy M. Spencer
  • I. J. Bruce

Abstract

Mating of haploid yeast strains can be done in a number of ways, either by mixing the yeast strains on a rich medium and isolating the resulting zygotes by micromanipulation, or by some variant of the drop-overlay method. The latter is frequently used for mating auxotrophic strains having complementary requirements. For prototrophic parental strains, of course, hybrids can only be isolated by micromanipulation of the zygotes from the mating mixture.

Keywords

Mating Type Haploid Strain Auxotrophic Strain Industrial Yeast Sterile Pipette 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Gunge N, Nakatomi N (1971) Genetics 70:41–58Google Scholar
  2. Spencer JFT, Spencer Dorothy M (1977) J Inst Brewing 83:287–289Google Scholar
  3. Palleroni N (1961) Phyton, Buenos Aires 16:117–128Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1989

Authors and Affiliations

  • John F. T. Spencer
    • 1
  • Dorothy M. Spencer
    • 1
  • I. J. Bruce
    • 2
  1. 1.Department of Life SciencesGoldsmith’s CollegeLondonGreat Britain
  2. 2.Department of Biology and Environmental HealthThames PolytechnicLondonGreat Britain

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