Rapid Isolation of DNA from Yeast

  • John F. T. Spencer
  • Dorothy M. Spencer
  • I. J. Bruce

Abstract

The method of Devenish and Newlon (1982) allows rapid, efficient isolation of yeast plasmid DNA. However, it does not aUow the recovery of high-molecular-weight DNA. On occasion it is useful to be able to obtain relatively small quantities of high-molecular-weight DNA rapidly for restriction analysis, Southern blotting, transformation etc. In such instances, it is laborious and time-consuming to follow the procedure given previously (p. 84). The method described below provides an alternative (Holm et al. 1986).

Keywords

Phenol EDTA Hydrochloride Sorbitol Sarcosyl 

Copyright information

© Springer-Verlag Berlin Heidelberg 1989

Authors and Affiliations

  • John F. T. Spencer
    • 1
  • Dorothy M. Spencer
    • 1
  • I. J. Bruce
    • 2
  1. 1.Department of Life SciencesGoldsmith’s CollegeLondonGreat Britain
  2. 2.Department of Biology and Environmental HealthThames PolytechnicLondonGreat Britain

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