The method of Devenish and Newlon (1982) allows rapid, efficient isolation of yeast plasmid DNA. However, it does not aUow the recovery of high-molecular-weight DNA. On occasion it is useful to be able to obtain relatively small quantities of high-molecular-weight DNA rapidly for restriction analysis, Southern blotting, transformation etc. In such instances, it is laborious and time-consuming to follow the procedure given previously (p. 84). The method described below provides an alternative (Holm et al. 1986).