Isolation of Pure High-Molecular-Weight DNA from the Yeast
The isolation of high molecular weight DNA from yeast depends upon: firstly, protoplasting or spheroplasting the cells and secondly, lysis and the differential separation of the DNA from other cell components. Generally the higher the percentage of cells sphero- or protoplasted, the higher the subsequent efficiency of DNA recovery. However, cells do not need to be fully protoplasted to give quite reasonable yields of DNA. The method which follows is based upon three published protocols and works well with all our laboratory strains of Saccharomyces cerevisiae.
KeywordsPhenol Lithium EDTA Saccharomyces Cohol
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