Isolation of Pure High-Molecular-Weight DNA from the Yeast

Saccharomyces cerevisiae
  • John F. T. Spencer
  • Dorothy M. Spencer
  • I. J. Bruce

Abstract

The isolation of high molecular weight DNA from yeast depends upon: first­ly, protoplasting or spheroplasting the cells and secondly, lysis and the dif­ferential separation of the DNA from other cell components. Generally the higher the percentage of cells sphero- or protoplasted, the higher the subse­quent efficiency of DNA recovery. However, cells do not need to be fully protoplasted to give quite reasonable yields of DNA. The method which fol­lows is based upon three published protocols and works well with all our laboratory strains of Saccharomyces cerevisiae.

Keywords

Phenol Lithium EDTA Saccharomyces Cohol 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1989

Authors and Affiliations

  • John F. T. Spencer
    • 1
  • Dorothy M. Spencer
    • 1
  • I. J. Bruce
    • 2
  1. 1.Department of Life SciencesGoldsmith’s CollegeLondonGreat Britain
  2. 2.Department of Biology and Environmental HealthThames PolytechnicLondonGreat Britain

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