Inhibition of Protein Synthesis and Amino Acid Transport by Dihydrotestosterone and 17ß-Estradiol in Chick Osteoblasts

  • H. Franck
  • F. van Valen
  • E. Keck
  • H. L. Krüskemper
Conference paper

Abstract

Although androgens and estrogens appear to be important regulators of skeletal growth, little is known about their direct effects on bone cells i.e. osteoblasts. Burnett et al. (1983) report about a significant increase in 3H-thymidine incorporation per µg DNA in bone. Canalis et al. (1978) showed that 17β-estradiol (E2) had a small and inconsistent stimulatory effect on the incorporation of 3H-proline into collagenase-digestible protein in rat calvariae and testosterone had no effect. In order to evaluate the potency of E2 and dihydrotestosterone (DHT) on protein synthesis and amino acid (AA) transport in osteoblast, we examined their effects in net AA uptake in chick osteoblasts (OB) on the 4th and 6th day in growing cultures.

Keywords

Estrogen Testosterone Proline Arginine Glutamine 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Burnett, C.C. and A.H. Reddi (1983): Influence of estrogen and progesterone on matrix-induced endochondral bone formation. Calcif. Tissue Int. 35, 609–614.PubMedCrossRefGoogle Scholar
  2. Canalis, E. and L.G. Raisz (1978): Effect of sex steroids on bone collagen synthesis in vitro. Calcif. Tiss. Res. 25, 105–110.CrossRefGoogle Scholar
  3. Lowry, O.H., N.R. Rosebrough, A.L. Farr, R.J. Randall (1951): Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193, 265.PubMedGoogle Scholar
  4. Riggs, T.R., M.W. Pan, H.W. Feng (1972): Transport of amino acids into the estrogen-primed uterus. J. Biol. Chem. 247, 7128–7134PubMedGoogle Scholar
  5. Spitzer, P.M. and G.F. Wassermann (1985): Amino acid uptake and protein synthesis in rat testes: Stimulation by dissociable factors. Horm. Metab. Res. 17, 237–240.CrossRefGoogle Scholar
  6. van Paassen, H.C., J. Portman, I.H.C. Borgart-Creutzburg, J.H.H. Thijssen, S.A. Duursma (1978): Oetrogen binding proteins in bone cell cytosol. Calcif. Tiss. Res. 25, 249–254.CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1987

Authors and Affiliations

  • H. Franck
  • F. van Valen
  • E. Keck
  • H. L. Krüskemper

There are no affiliations available

Personalised recommendations