Inhibition of Protein Synthesis and Amino Acid Transport by Dihydrotestosterone and 17ß-Estradiol in Chick Osteoblasts
Although androgens and estrogens appear to be important regulators of skeletal growth, little is known about their direct effects on bone cells i.e. osteoblasts. Burnett et al. (1983) report about a significant increase in 3H-thymidine incorporation per µg DNA in bone. Canalis et al. (1978) showed that 17β-estradiol (E2) had a small and inconsistent stimulatory effect on the incorporation of 3H-proline into collagenase-digestible protein in rat calvariae and testosterone had no effect. In order to evaluate the potency of E2 and dihydrotestosterone (DHT) on protein synthesis and amino acid (AA) transport in osteoblast, we examined their effects in net AA uptake in chick osteoblasts (OB) on the 4th and 6th day in growing cultures.
KeywordsAmino Acid Transport Medium Replacement Isoleucine Leucine Lysine Histidine Tyrosine phenYlalanine
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