Abstract
We have applied the method of DNA polymorphism analysis to resolve paternity cases. DNA samples were isolated from the blood of mother, child, and alleged father, digested with the restriction endonuclease Pst 1, size separated by agarose gel electrophoresis, and hybridized with four recombinant DNA probes which recognize hypervariable regions in the human genome. Determination of DNA fragment sizes was accomplished with a computer assisted digitizing system. Co-migration of paternally derived DNA fragments in the child and alleged father indicated possible paternity, and a paternity index (PI) was calculated from allele frequency tables accumulated for each polymorphic locus. The cumulative power of exclusion with these four probes, for American Blacks and Caucasians, was on average 99.5% and the mean paternity index more than 3600.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Botstein, D., White, R. L., Skolnick, M. and Davis, R. W., “Construction of a Genetic Linkage Map in Man Using Restriction Fragment Polymorphisms”, American Journal of Human Genetics, vol. 32, 1980, pp. 314–331.
Baird, M., Balazs, I., Giusti, A., Miyazaki, L., Nicholas, L., Wexler, K., Kanter, E., Glassberg, J., Allen, F., Rubinstein, P., and Susman, L., “Allele Frequency Distribution of Two Highly Polymorphic DNA Sequences in Three Ethnic Groups and Its Application to the Determination of Paternity”, American Journal of Human Genetics. vol. 39, 1986, pp. 489–501.
Nakamura, Y., Leppert, M., O’Connell, P., Wolff, R., Holm, T., Culver, M., Martin, C., Fujimoto, E., Holff, M., Kumlin, E. and White, R., “Variable Number of Tandem Repeat (VNTR) Markers for Human Gene Mapping”, Science, vol. 235, 1987, pp. 1616–1622.
Feinberg, A. P. and Vogelstein, B., “A Technique for Radiolabelling DNA Restriction Endonuclease Fragments to High Specific Activity”, Analytical Biochemistry, vol. 137, 1983, pp. 6–13.
Elder, J. K. and Southern, E. M., “Measurement of DNA Length by Gel Electrophoresis. II: Comparison of Methods for Relating Mobility to Fragment Length”, Analytical Biochemistry, vol. 128, 1983, pp. 227–231.
Ito, H., Yasuda, N. and Matsumoto, H., “The Probability of Parentage Exclusion Based on Restriction Fragment Length Polymorphisms”, Jpn. J. Human Genet, vol. 30, 1985, pp. 261–269.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1988 Springer-Verlag Berlin Heidelberg
About this paper
Cite this paper
Baird, M. et al. (1988). The Application of DNA-Print for the Estimation of Paternity. In: Mayr, W.R. (eds) Advances in Forensic Haemogenetics. Advances in Forensic Haemogenetics, vol 2. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-73330-7_66
Download citation
DOI: https://doi.org/10.1007/978-3-642-73330-7_66
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-18765-3
Online ISBN: 978-3-642-73330-7
eBook Packages: Springer Book Archive