The Application of DNA-Print for the Estimation of Paternity

  • M. Baird
  • K. Wexler
  • M. Clyne
  • E. Meade
  • L. Ratzlaff
  • G. Smalls
  • P. Benn
  • J. Glassberg
  • I. Balazs
Part of the Advances in Forensic Haemogenetics book series (HAEMOGENETICS, volume 2)

Abstract

We have applied the method of DNA polymorphism analysis to resolve paternity cases. DNA samples were isolated from the blood of mother, child, and alleged father, digested with the restriction endonuclease Pst 1, size separated by agarose gel electrophoresis, and hybridized with four recombinant DNA probes which recognize hypervariable regions in the human genome. Determination of DNA fragment sizes was accomplished with a computer assisted digitizing system. Co-migration of paternally derived DNA fragments in the child and alleged father indicated possible paternity, and a paternity index (PI) was calculated from allele frequency tables accumulated for each polymorphic locus. The cumulative power of exclusion with these four probes, for American Blacks and Caucasians, was on average 99.5% and the mean paternity index more than 3600.

Keywords

Agarose Electrophoresis Nylon 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1988

Authors and Affiliations

  • M. Baird
    • 1
  • K. Wexler
    • 1
  • M. Clyne
    • 1
  • E. Meade
    • 1
  • L. Ratzlaff
    • 1
  • G. Smalls
    • 1
  • P. Benn
    • 1
  • J. Glassberg
    • 1
  • I. Balazs
    • 1
  1. 1.Lifecodes CorporationElmsfordUSA

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