The Application of Monoclonal Antibodies for the Definition of Genetic Markers of Human Red Cells

  • D. Voak
  • D. Davies
  • H. Sonneborn
  • J. Moulds
  • A. Fletcher
  • D. M. Downie
Part of the Advances in Forensic Haemogenetics book series (HAEMOGENETICS, volume 2)


Monoclonal antibodies have now been produced to the red cell antigens A, A1, B, A and B, M, N, D, LW, e, H, I, Lea, Leb, P, P1, K, k, LKE, Lub, T, Tn, Wrb, and MER 2.


Blood Group Antigen Saline Test Monoclonal Antibody Reagent Saline Titre Trypsin Chymotrypsin 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Anstee DJ (1983), The structure of the MN and Ss blood group antigens. Bio Bull 4: 318–321.Google Scholar
  2. Bird GWG, Wingham J (1970) N-acetyl neuraminic (sialic) acid and human blood group antigen structure. Vox Sang 18: 240–243.PubMedCrossRefGoogle Scholar
  3. Bron D, Feinberg MB, Nelson NHT, Kaplan HS (1984) Production of human monoclonal IgG antibodies against Rhesus (D) antigen. Proc. Nat. Acad. Sci. USA 81: 3214–3217.PubMedCrossRefGoogle Scholar
  4. Crawford DH, Barlow MJ, Harrison JF, Winger L, Huehns ER (1983) Production of human antibody to Rhesus D antigen. Lancet 1: 386–388.PubMedCrossRefGoogle Scholar
  5. Dahr W, Uhlenbruck G, Knott H (1975) Immunochemical aspects of the MNSs-blood group system. J of Imm 2: 87–100.CrossRefGoogle Scholar
  6. Dahr W, Uhlenbruck G, Jansen E, Schmalish R (1977) Different N-terminal amino acids in the MN glycoprotein from MM and NN erythrocytes. Human Genet 35: 335–343.CrossRefGoogle Scholar
  7. Dahr W, Uhlenbruck G, Leikola J, Wagstaff W (1978) Studies on the membrane glycoprotein defect of En (a-) erythrocytes: III N-terminal amino acids of sialoglyoproteins from normal and En (a-) red cells. J of Imm 5: 117–123.CrossRefGoogle Scholar
  8. Fletcher A, Harbour C, Matthews M, de Zwart R, Ford D (1986) Blood grouping with monoclonal anti-N antibodies. Aus J of Exp Biol & Med Sci 64: 215–222.CrossRefGoogle Scholar
  9. Frazer RH, Munro AC, Williamson AR, Barrie EK, Hamilton EA, Mitchell R (1982) Mouse monoclonal anti-N. J of Immun 9: 295–302.CrossRefGoogle Scholar
  10. Greenwell P, Yates AD, Watkins WM (1979) Blood group A synthesising activity of the blood group B gene specified-3-galactosyl transferase. In Glyconjugates, eds R Schaur, P Boer, E Buddecke, MF Kramer. Vliegenthart and Wiegendt, Thieme, Stuttgart. 268–269.Google Scholar
  11. Hoppe AH (1979) Considerations in the selection of reagents. American Association of Blood Banks, Washington, Appendix 1, p 15.Google Scholar
  12. Kohler G, Milstein C (1975) Continuous cultures of fused cells secreting antibody of predefined specificity. Nature 256: 495–497.PubMedCrossRefGoogle Scholar
  13. Lowe AD, Lennox E, Voak D (1984) A new monoclonal anti-A: culture supernatants with the performance of hyperimmune human reagents. Vox Sang 46: 29–35.PubMedCrossRefGoogle Scholar
  14. Lowe AD, Green SM, Voak D et al (1986) A human-human monoclonal anti-D by direct fusion with a lymphoblastoid line. Vox Sang 51 : 212–216.PubMedCrossRefGoogle Scholar
  15. Messeter L, Brodin T, Chester MA et al (1984) Mouse monoclonal antibodies with anti-A, anti-B and anti-A, B specificities; some superior to human polyclonal ABO reagents. Vox Sang 46: 185–194.PubMedCrossRefGoogle Scholar
  16. Moore BPL (1984) Does knowledge of Du status serve a useful purpose. Vox Sang 46: 95–102.CrossRefGoogle Scholar
  17. Moore S, Chirnside A, Micklem LR et al (1984) A mouse monoclonal antibody with anti-A(B) specificity which agglutinates A cells. Vox Sang 47: 427–434.PubMedCrossRefGoogle Scholar
  18. Nichols ME, Rosenfield RE, Rubinstein P (1985) Two blood group M epitopes disclosed by monoclonal antibodies. Vox Sang 49: 134–148.CrossRefGoogle Scholar
  19. Race RR, Sanger R (1975) Blood groups in Man. 6th edn. Blackwell Oxford, pp 189–193.Google Scholar
  20. Rubocki R, Milgrom F (1986) Reactions of murine monoclonal antibodies to blood group MN antigens. Vox Sang 51: 217–225PubMedCrossRefGoogle Scholar
  21. Sacks S, Lennox E (1981) Monoclonal anti-B as a new source of blood typing reagents. Vox Sang 40: 99–104.PubMedCrossRefGoogle Scholar
  22. Sonneborn HH, Uthemann H, Munro AC, Bruce M, Fraser RH, Inglis G (1984) Reactivity of monoclonal antibodies directed against blood group antigens M and N. Dev Biol Stand 57: 61–68.PubMedGoogle Scholar
  23. Sonneborn HH, Ernst M (1987) Further characterisation and standardisation of mouse monoclonal antibodies reacting with M/N blood group antigens. Dev Biol Stand 67: 97–104.PubMedGoogle Scholar
  24. Thompson KM, Melamed MD, Eagle K, et al (1986) Production of human monoclonal IgG and IgM antibodies with anti-D rhesus specificity using heterohybridomas. Imm 58: 157–160.Google Scholar
  25. Tippett P, Sanger R (1962) Observations on subdivisions of the Rh antigen D, Vox Sang 7: 9–13.PubMedCrossRefGoogle Scholar
  26. Treacy M, Stroup M (eds) (1987) Proceedings of A scientific forum on blood grouping anti-A (murine monoclonal blend) Bioclone. Sept 1986, Raritan. Ortho Diagnostics DS1 – 346.Google Scholar
  27. Voak D (1986) Monoclonal antibodies: application to blood group serology. Labmed Aug/Sept: 27–31.Google Scholar
  28. Voak D, Lowe AD, Lennox E (1983) Monoclonal antibodies: ABO serology Bio Bull 4: 291–299.Google Scholar
  29. Voak D, Lennox E (1986) Monoclonal antibodies for laboratory aspects of transfusion practice. Progress in Transfusion Medicine 1: ed J. Cash, pp 1–18. Churchill Livingstone, Edinburgh.Google Scholar
  30. Voak D, Davies D, Beck M, Yates AD (1987) (to be published) The B(A) phenomenon: a monoclonal anti-A (MHO4) demonstrates traces of “A” on normal group B red cells. Vox Sang.Google Scholar
  31. Wasniowska K, Reichert CM, McGinniss MH et al (1985) Two monoclonal antibodies highly specific for the blood group N determinant. Glyconjugate J 2: 163–176.CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1988

Authors and Affiliations

  • D. Voak
    • 1
  • D. Davies
    • 1
    • 2
  • H. Sonneborn
    • 1
    • 3
  • J. Moulds
    • 1
    • 4
  • A. Fletcher
    • 1
    • 5
  • D. M. Downie
    • 1
  1. 1.Regional Transfusion and Immuno-Haematology CentreCambridgeUK
  2. 2.Ortho DiagnosticsRaritanUSA
  3. 3.Biotest-Serum- InstituteFrankfurtGermany
  4. 4.Gamma BiologicalsHoustonUSA
  5. 5.Red Cross Blood Transfusion ServiceSydneyAustralia

Personalised recommendations