Polymorphism of AHS-Glycoprotein: Comparison of different IEF and detection techniques

  • C. Luckenbach
  • J. Kömpf
  • H. Ritter
Part of the Advances in Forensic Haemogenetics book series (HAEMOGENETICS, volume 2)

Abstract

The human plasma protein alpha-2-HS-glycoprotein (A2HS, Schultze et al. 1962) was first described separately by Heremans (1960) and Schmid and Bürgi (1961). A2HS consists of one polypeptide chain with a molecular weight of 49000 D. The content of carbohydrate amounts to 13.4% (Schmid/Bürgi 1961) and of sialic acids to 4.1% (Putnam 1975). Lewis/Andre 1978).

Keywords

Cellulose Glycerol Hydrate Albumin Urea 

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References

  1. Cox D W, Andrews B J (1982) Silver stain immunofixation for alpha-2-HS-glycoprotein. A new method for detection of protein heterogeneity. Electrophoresis, edited by Stathakos D, Berlin, W de Gryter 1983: 243–247Google Scholar
  2. Eiberg H, Mohr J, Nielsen L S (1984) Alpha-2-HS: new methods of phenotyping and analysis of linkage relations: assignement to chromosome 3. Human Gene Mapping 7 (Los Angeles Conference 1983): 461Google Scholar
  3. Heremans J F (1960) Les Globulines Sériques du Systeme Gamma. Arscia, Brüssel: 103–105Google Scholar
  4. Klett C (1985) Der genetische Polymorphismus des Alpha-2-HS-Glycoproteins: Methodik und Genetik. Biol. Diplomarbeit, TübingenGoogle Scholar
  5. Putnam F W (1975) The Plasma Proteins. Ed F W Putnam 2nd ed, Academic Press New York: 1–55Google Scholar
  6. Schmid K, Bürgi W (1961) Preparation and properities of the human plasma Ba-alpha2-glycoprotein. Biochim Biophys Acta 47: 440–453PubMedCrossRefGoogle Scholar
  7. Schultze H E, Heide K, Haupt H (1962) Charakterisierung eines niedermolekularen alpha-2-Mukoids aus Humanserum. Naturwissenschaften 49: 15CrossRefGoogle Scholar
  8. Umetsu K, Kashimura S, Ikeda N, Suzuki Y (1984) A new alpha-2-HS-glycoprotein typing by isoelectric focusing. Hum Genet 67: 70–71PubMedCrossRefGoogle Scholar
  9. Umetsu K, Yuasa J, Suzuki T (1986) The polymorphism of desialyzed A2HS-glycoprotein (AHS): Isoelectric focusing in 2,5 M urea as a method for identification of genetic variants. Hum Genet 73: 372–373PubMedCrossRefGoogle Scholar
  10. Weidinger S, Schwarzfischer F, Burgemeister R, Cleve H (1984) Genetic alpha-2-HS-glycoprotein phenotypes demonstrated by isoelectric focusing and immunofixation. Electrophoresis ’84 ed by Neuhoff V Weinheim West-Germany Verlag Chemie: 487–4901.Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1988

Authors and Affiliations

  • C. Luckenbach
    • 1
  • J. Kömpf
    • 1
  • H. Ritter
    • 1
  1. 1.Institut für Anthropologie und Humangenetik der Universität TübingenTübingenGermany

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