Classification of alphal antitrypsin phenotypes by high-resolution two-dimensional electrophoresis (2-D PAGE)

  • V. L. Pascali
  • E. d’Aloja
  • M. Dobosz
Part of the Advances in Forensic Haemogenetics book series (HAEMOGENETICS, volume 2)

Abstract

The ability of two-dimensional gel electrophoresis (2-D PAGE) to visualize soluble proteins is well documented (Anderson and Anderson 1977). By this tecnique, exploiting charge/mass differences to separate polypeptides, many polymorphic serum proteins were exactly located in 2-D PAGE maps of human serum (Anderson and Anderson 1979). The main advantages of this procedure are: first, a better molecular characterization of mutants is obtainable, secondly a simultaneous detection of several proteins is possible in the same slab gel;finally, the monitoring of non-conventional phenotypes,as the null variants (which would otherwise be difficult to detect) could be obtained (Asakawa et al. 1985).

Keywords

Glycerol Urea Carbohydrate Agarose Electrophoresis 

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References

  1. Anderson NL, Anderson NG (1977) High resolution twodimensional electrophoresis of human plasma proteins. Proc Natl Acad Sci USA 74: 5421–5425.PubMedCrossRefGoogle Scholar
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Copyright information

© Springer-Verlag Berlin Heidelberg 1988

Authors and Affiliations

  • V. L. Pascali
    • 1
  • E. d’Aloja
    • 1
  • M. Dobosz
    • 1
  1. 1.Immunohematology laboratory, Istituto di Medicina LegaleUniversità Cattolica del Sacro CuoreRomaItaly

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