Classification of alphal antitrypsin phenotypes by high-resolution two-dimensional electrophoresis (2-D PAGE)
The ability of two-dimensional gel electrophoresis (2-D PAGE) to visualize soluble proteins is well documented (Anderson and Anderson 1977). By this tecnique, exploiting charge/mass differences to separate polypeptides, many polymorphic serum proteins were exactly located in 2-D PAGE maps of human serum (Anderson and Anderson 1979). The main advantages of this procedure are: first, a better molecular characterization of mutants is obtainable, secondly a simultaneous detection of several proteins is possible in the same slab gel;finally, the monitoring of non-conventional phenotypes,as the null variants (which would otherwise be difficult to detect) could be obtained (Asakawa et al. 1985).
KeywordsGlycerol Urea Carbohydrate Agarose Electrophoresis
Unable to display preview. Download preview PDF.