Enzymdarstellung am fixierten Organgewebe

  • E. Tutsch-Bauer
  • E. Josephi
  • J. Teifel-Greding
  • W. Spann
Conference paper
Part of the Advances in Forensic Haemogenetics book series (HAEMOGENETICS, volume 2)

Abstract

The successful determination of GLO subtypes (starch gel electrophoresis) and PGM subtypes (isoelectrofocusing) in tissues, skeletal musculature and liver, applying ethanol and acetone fixation is reported.

Other fixing agents such as formalin, buffered formalin pH 7, 5 and glutaraldehyde are destroying any enzyme activity of the considered systems EsD, GLO, GPT, SEP, PGM.

Applying ethanol and acetone fixation no results could be obtained in EsD and SEP system.

Differing results showed GPT, the intensity of the spots varied extremely even under stable experimental conditions.

GLO and PGM subtyping using ethanol was successful within a period of four weeks. PGM subtypes using acetone fixation could be demonstrated even after a storage time of two months.

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Literatur

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Copyright information

© Springer-Verlag Berlin Heidelberg 1988

Authors and Affiliations

  • E. Tutsch-Bauer
    • 1
  • E. Josephi
    • 1
  • J. Teifel-Greding
    • 1
  • W. Spann
    • 1
  1. 1.Institut für RechtsmedizinUniversität MünchenMünchen 2Germany

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