Transforming Growth Factor-α
Transforming growth factor-α (TGF-α) was first detected in the culture medium of certain retrovirus-transformed cell lines as an activity which binds to the receptor for epidermal growth factor (EGF) and thereby inhibits the binding of EGF. Subsequent examination showed that this factor is made by many other transformed cell lines but not by adult normal cells in culture (Todaro et al. 1985). Addition of sarcoma growth factor, as it was first called, to rat fibroblasts of the NRK cell line reversibly induced profound morphological changes and colony-forming ability in soft agar (De Larco and Todaro 1978; Todaro et al. 1985). The capacity of this factor to confer a transformed phenotype upon NRK cells, together with the fact that it is synthesized by transformed cells, led to the name transforming growth factor.
KeywordsAgar Tyrosine Cysteine Carboxyl Sarcoma
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- Derynck R, Goeddel DV, Ullrich A, Gutterman JU, Williams R, Bringman TS, Berger WH (1986) Synthesis of mRNAs for the transforming growth factors-α and -β and the epidermal growth factor receptor by human tumors. SubmittedGoogle Scholar
- Marquardt H, Hunkapiller MW, Hood LE, Twardzik DR, De Larco JE, Stephenson JR, Todaro GJ (1983) Transforming growth factors produced by retrovirus-transformed rodent fibroblasts and human melanoma cells: Amino acid sequence homology with epidermal growth factor. Proc Natl Acad Sci USA 80: 4684–4688CrossRefPubMedGoogle Scholar
- Myrdal S (1985) Differences in early cellular responses to epidermal growth factor and transforming growth factor, type-alpha. J Cell Biol 101: 2440Google Scholar
- Todaro GJ, Lee DC, Webb NR, Rose TM, Brown JP (1985) Rat type-a transforming growth factor: Structure and possible function as a membrane receptor: In: Feramisco J, Ozanne B, Stiles C (eds) Cancer cells 3. Cold Spring Harbor Lab, Cold Spring Harbor, pp 51–58Google Scholar