A Histological Evaluation of Experimental Nerve Anastomoses with the 1.32-μm Nd:YAG Laser
The use of the laser in experimental nerve anastomoses is a procedure already established for various types of instruments (1, 3, 9) In a previous investigation using the 1.32-μm Nd:YAG-laser, the welding property could be attributed to a coagulative alteration of collagen with ultrastructural features of fusion of individual fibrils (7). The formation of suture granulomas is thus avoided while, according to a study with the CO2 laser (4), laser-induced damage to the nerve can be kept minimal. Although the regeneration potential apparently remains undiminished, the distal myelinated nerve fiber population was found not to be significantly different between CO2 laser-assisted and traditional suture anastomoses in a quantitative analysis (2) However, a detailed and systematic study of the changes taking place at the site of anastomosis is as yet lacking, probably due to technical difficulties inherent in the histological preparation of longer nerve segments. We have tried to overcome these by use of a new plastic embedding technique.
KeywordsDioxide Welding Argon Epoxy Dehydration
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