A Histological Evaluation of Experimental Nerve Anastomoses with the 1.32-μm Nd:YAG Laser

  • R. Schober
  • F. Ulrich
  • Th. Sander
Conference paper
Part of the Advances in Neurosurgery book series (NEURO, volume 16)


The use of the laser in experimental nerve anastomoses is a procedure already established for various types of instruments (1, 3, 9) In a previous investigation using the 1.32-μm Nd:YAG-laser, the welding property could be attributed to a coagulative alteration of collagen with ultrastructural features of fusion of individual fibrils (7). The formation of suture granulomas is thus avoided while, according to a study with the CO2 laser (4), laser-induced damage to the nerve can be kept minimal. Although the regeneration potential apparently remains undiminished, the distal myelinated nerve fiber population was found not to be significantly different between CO2 laser-assisted and traditional suture anastomoses in a quantitative analysis (2) However, a detailed and systematic study of the changes taking place at the site of anastomosis is as yet lacking, probably due to technical difficulties inherent in the histological preparation of longer nerve segments. We have tried to overcome these by use of a new plastic embedding technique.


Dioxide Welding Argon Epoxy Dehydration 


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  1. 1.
    Almquist EE, Nachemson A, Auth D, Almquist B, Hall S (1984) Evaluation of the use of the argon laser in repairing rat and primate nerves. J Hand Surg (Am) 9:792–799Google Scholar
  2. 2.
    Beggs JL, Fischer DW, Shetter AG (1986) Comparative study of rat sciatic nerve microepineurial anastomoses made with carbon dioxide laser and suture techniques. Part 2. A morphometric analysis of myelinated nerve fibers. Neurosurgery 18:266–269PubMedCrossRefGoogle Scholar
  3. 3.
    Fischer DW, Beggs JL, Kenshalo DL Jr, Shetter AG (1985) Comparative study of microepineurial anastomoses with the use of CO2 laser and suture techniques in rat sciatic nerves: Part 1. Surgical technique, nerve action potentials, and morphologic studies. Neurosurgery 17:300–308PubMedCrossRefGoogle Scholar
  4. 4.
    Myers RR, James HE, Powell HC (1985) Laser injury of peripheral nerve: a model for focal endoneurial damage. J Neurol Neurosurg Psychiat 48:1265–1268PubMedCrossRefGoogle Scholar
  5. 5.
    Richmond IL (1986) The use of lasers in nerve repair. In: Fasano AV (ed) Advanced intraoperative technologies in neurosurgery. Springer, Wien New York, pp 175–183Google Scholar
  6. 6.
    Samii M, Wallenborn R (1972) Tierexperimentelle Untersuchungen über den Einfluß der Spannung auf den Regenerationserfolg nach Nervennaht. Acta Neurochir 27:87–110CrossRefGoogle Scholar
  7. 7.
    Schober R, Ulrich F, Sander T, Dürselen H, Hessel S (1986) Laser-induced alteration of collagen substructure allows microsurgical tissue welding. Science 232:1421–1422PubMedCrossRefGoogle Scholar
  8. 8.
    Schröder JM, Seiffert KE (1970) Die Feinstruktur der neuromatösen Neurotisation von Nerventransplantaten. Virchows Arch (Abt B) 5: 219–235Google Scholar
  9. 9.
    Ulrich F, Sander T, Bock WJ (1986) Anastomosis of the sciatic nerve of the rat with the modified Nd:YAG laser. A preliminary report. In: Waidedelich W, Kiefhaber P (eds) Laser/Optoelektronik in der Medizin. Springer, Berlin Heidelberg New York Tokyo, pp 414–417Google Scholar
  10. 10.
    Ulrich R, Schober R, Bock WJ (this volume) Laser-Assisted Micro-anastomoses.Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1988

Authors and Affiliations

  • R. Schober
    • 1
  • F. Ulrich
    • 1
  • Th. Sander
    • 1
  1. 1.Neuropathologisches Institut der UniversitätDüsseldorf 1Germany

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