Analysis of the Structure, Organization and Role of Cytoskeleton During Pollen Germination and Tube Growth in Pyrus communis L.
Behavior of actin microfilaments and microtubules was followed in the vegetative cell of germinating pollen and pollen tubes of pyrus communis using rhodamine-conjugated phalloidin to visualize actin, indirect immunofluorescence to localize microtubules, and conventional and freeze-substitution electron microscopy. Several previous investigations have focused on the cytoskeleton of pollen tubes at the ultrastructural level (Franke et al., 1972; Miki-Hirosige & Nakamura, 1982; Derksen et al., 1985; Tiezzi et al., 1986 Lancelle et al., 1987) and at the light microscope level (Derksen et al., 1985; Perdue & Parthasarathy, 1985; Pierson et al., 1985; Tiezzi et alata., 1986) using fluorescent probes. However, questions remain about the precise organization of the pollen tube cytoskeleton, and there has been scant attention to dynamics of cytoskeletal elements during the periods of pollen hydration and activation that precede germination. Pollen Activation. By using the actin-specific probe, rhodamineconjugated phalloidin (RP), to study the dynamics of actin microfilaments, we identified several stages of actin organization as dehydrated pollen grains become activated and eventually germinate.
KeywordsPollen Tube Pollen Germination Cortical Microtubule Actin Microfilament Vegetative Nucleus
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- Derksen J, Pierson ES, Traas JA (1985) Microtubules in vegetative and generative cells of pollen tubes. Eur. J. Cell Biol. 38: 142Google Scholar
- Derksen J, Traas JA (1985) Growth of tobacco pollen tubes in vitro; effects of drugs interfering with the cytoskeleton. In: Willemse MTM, Van Went JL (eds) Proceedings, 8th International Symposium on Sexual Reproduction in Seed Plants, Ferns, and Mosses. Pudoc, Wageningen, p 64Google Scholar
- Miki–Hirosige H, Nakamura S (1982) Process of metabolism during pollen tube wall formation. J. Electron Microscopy 31: 51–62Google Scholar
- Perdue TD, Parthasarathy, MV (1985) In situ localization of F–actin in pollen tubes. Eur. J. Cell Biol. 39: 13–20Google Scholar
- Pierson ES, Derksen J, Traas JA (1986) Organization of microfilaments and microtubules in pollen tube grown in vitro, or in vivo in various angiosperms. Eur. J. cell Biol. 41: 14–18.Google Scholar
- Tiezzi A, Cresti M, ciampolini F (1986) Microtubules in Nicotiana pollen tubes: ultrastructural, immunofluorescence and biochemical data. In: Cresti M, Dallai R (eds) Biology of Reproduction and Cell Motility in Plants and Animals. University of Siena, p 87Google Scholar