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31P and 1H NMR as a Non-Destructive Method for Measuring Pollen Viability

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Abstract

Most techniques that provide information about the viability of pollen, e.g. staining with vital dyes or germinability tests, are destructive and thus of limited value where pollen supplies are small and the pollen is required for pollination or destructive biochemical experiments. Dumas et al. (1982) have reported using proton (1H) nuclear magnetic resonance (NMR) to correlate water content with pollen viability. Another method for estimating pollen viability would be to use phosphorus (31P) NMR which provides information as to the amounts of organic phosphorus (e.g., ATP) available for metabolism.

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References

  • Dumas C, Duplan J, Said C, Soulier J (1983) 1H Nuclear magnetic resonance to correlate water content and pollen viability. In: Mulcahy DL, Ottoviano E (Eds) Pollen: Biology and Implications for Plant Breeding. Elsevier, Amsterdam, p15

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  • Duplan J, Dumas C (1984) Viabilite Pollinique et Conservation du Pollen. In: Herve Y., Dumas C (Eds) Incompatibilite Pollinique et Amelioration des Plantes. Le Departement de Formation Continue de I’Ecole Nationale Superieure Agronomique, Rennes Cedex, France, p 40

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  • Priestly DA, de Kruijff B (1982) Phospholipid motional characteristics in a dry biological system. Plant Physiol. 70: 1075–1078

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© 1988 Springer-Verlag Berlin Heidelberg

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Ladyman, J.A.R., Taylor, R.E. (1988). 31P and 1H NMR as a Non-Destructive Method for Measuring Pollen Viability. In: Cresti, M., Gori, P., Pacini, E. (eds) Sexual Reproduction in Higher Plants. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-73271-3_11

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  • DOI: https://doi.org/10.1007/978-3-642-73271-3_11

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-73273-7

  • Online ISBN: 978-3-642-73271-3

  • eBook Packages: Springer Book Archive

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