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Cholinergic-Specific Antigens

  • Chapter
The Cholinergic Synapse

Part of the book series: Handbook of Experimental Pharmacology ((HEP,volume 86))

Abstract

In recent years immunochemical techniques have begun to play a much larger role in neurobiology than formerly. They have been applied in various ways and for different purposes. Antibodies raised against components specific for a particular type of nerve cell may be used to trace its location and connections within the nervous system. Antibodies raised against known transmitter-specific components may be used to trace pathways utilizing neurons of this particular transmitter type. In the cholinergic system, choline acetyltransferase (ChAT) is the obvious component to select, and both polyclonal and monoclonal antibodies which are specific for ChAT are now available. In Chap. 22 the results of such studies are reviewed. Such methods have tended to replace histochemical methods which rely on a chemical reation; thus methods based on ChAT immunohistochemistry have tended to displace older methods which detected acetylcholinesterase (AChE), in itself not always a reliable cholinergic marker, by precipitating the thioacetate generated by the enzymic hydrolysis of acetylthiocholine as the copper salt.

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Authors
  1. V. P. Whittaker
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  2. E. Borroni
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Editor information

Editors and Affiliations

  1. Arbeitsgruppe Neurochemie, Max-Planck-Institut für biophysikalische Chemie, Postfach 2841, D-3400, Göttingen, Germany

    Victor P. Whittaker

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© 1988 Springer-Verlag Berlin Heidelberg

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Whittaker, V.P., Borroni, E. (1988). Cholinergic-Specific Antigens. In: Whittaker, V.P. (eds) The Cholinergic Synapse. Handbook of Experimental Pharmacology, vol 86. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-73220-1_16

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  • DOI: https://doi.org/10.1007/978-3-642-73220-1_16

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-73222-5

  • Online ISBN: 978-3-642-73220-1

  • eBook Packages: Springer Book Archive

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