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Generation and Measurement of Antibodies

  • Chapter
The Pharmacology of Lymphocytes

Part of the book series: Handbook of Experimental Pharmacology ((HEP,volume 85))

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Abstract

The antibody (Ab) molecule has evolved to perform two distinct functions: epitope recognition and elimination of foreign antigens (Ag). Ab activity resides in immunoglobulin (Ig) molecules, each of which can interact with some of a virtually unlimited number of physicochemically different Ags. These Ags include exogenous (foreign or nonself) as well as endogenous (self) Ags, including Ig idiotopes (Eichmann 1978; Jerne 1984; Köhler et al. 1984). In spite of the large variety of different Ab specificities, Ab destroy or eliminate foreign Ag by a small number of effector mechanisms. To carry out its dual function, the Ab molecule has evolved discrete globular domains: one of these domains binds Ag, whereas the others mediate effector mechanisms (Edelman 1973). Thus, the functional duality of the Ab molecule is reflected in its three-dimensional structure. The organization of Ab-gene clusters also reflects this duality (Honjo 1983; Calvert et al. 1984).

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  1. H. F. J. Savelkoul
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  2. S. S. Pathak
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  3. N. R. Sabbele
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  4. R. Benner
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  1. Research Department, CIBA-GEIGY AG, CH-4002, Basel, Switzerland

    Michael A. Bray Ph. D.

  2. Pharmaceutical Division, Preclinical Research, SANDOZ Ltd., CH-4002, Basel, Switzerland

    John Morley Ph. D., FRC Path.

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Savelkoul, H.F.J., Pathak, S.S., Sabbele, N.R., Benner, R. (1988). Generation and Measurement of Antibodies. In: Bray, M.A., Morley, J. (eds) The Pharmacology of Lymphocytes. Handbook of Experimental Pharmacology, vol 85. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-73217-1_6

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