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The Pharmacology of Lymphocytes pp 319–343Cite as

Lymphocyte Purification, Growth, Cloning and Functional Assays

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Part of the book series: Handbook of Experimental Pharmacology ((HEP,volume 85))

Abstract

Lymphocytes comprise an extremely heterogeneous mixture of cells, differing in both specificity and function. Thus the immune system can recognise and respond to an enormous range of antigens and can mount functionally distinct types of responses to different forms of antigen (Chap. 1). The classical reductionalist approach to the problem of lymphocyte heterogeneity has been to obtain homogeneous populations of cells. In this way the signals acting on different populations of cells and the consequences of such signals can be determined. Homogeneous populations of cells have been prepared by either the extensive purification of mixed populations of cells or, more rigorously, by the growth and cloning of single cells from a mixed population.

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Authors
  1. M. J. H. Ratcliffe
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  2. J. R. Lamb
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Editors and Affiliations

  1. Research Department, CIBA-GEIGY AG, CH-4002, Basel, Switzerland

    Michael A. Bray Ph. D.

  2. Pharmaceutical Division, Preclinical Research, SANDOZ Ltd., CH-4002, Basel, Switzerland

    John Morley Ph. D., FRC Path.

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© 1988 Springer-Verlag Berlin Heidelberg

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Ratcliffe, M.J.H., Lamb, J.R. (1988). Lymphocyte Purification, Growth, Cloning and Functional Assays. In: Bray, M.A., Morley, J. (eds) The Pharmacology of Lymphocytes. Handbook of Experimental Pharmacology, vol 85. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-73217-1_13

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  • DOI: https://doi.org/10.1007/978-3-642-73217-1_13

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-73219-5

  • Online ISBN: 978-3-642-73217-1

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