Stage-Specifically Expressed Antigens and Genes in Trypanosoma cruzi

  • G. Harth
  • C. G. Haidaris
  • M. So
Part of the Colloquium der Gesellschaft für Biologische Chemie 9.–11. April 1987 in Mosbach/Baden book series (MOSBACH, volume 38)

Abstract

The parasitic protozoan Trypanosoma cruzi is the causative agent of Chagas’ disease which affects some 20–30 million people in Central and South America [1]. During its life cycle, the parasite undergoes a number of morphologic and physiologic changes as it passes between two hosts, a reduviid bug and man. Epimastigote or noninfectious forms are found in the midgut of the insect. In the hindgut they change to infectious metacyclic trypomastigotes which are deposited, via the insect feces, on an individual’s skin. Soon after entry through a break in the skin or via a mucous membrane, the trypomastigotes invade host cells, replicate as amastigotes, and convert back to trypomastigotes. When the cell ruptures, these forms are released in the bloodstream. Here, they either spread to surrounding tissues or enter the circulation and are taken up by the hematophagous reduviid. In the insect’s gut, they convert to replicating epimastigotes and the cycle is completed [1]. The most striking change occurs as epimastigotes convert to trypomastigotes. In contrast to epimastigotes, trypomastigotes are infectious to man, do not replicate, are resistant to complement mediated lysis and express high levels of neuraminidase [1–4]. The basis for these metabolic and antigenic changes is directly linked to differential gene expression.

Keywords

Cellulose Dodecyl Galactose Hydroxyapatite Formamide 

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References

  1. 1.
    De Souza W (1984) Cell biology of Tvypanosoma cruzi. Int Rev Cytol 86:197–283PubMedCrossRefGoogle Scholar
  2. 2.
    Sher A, Hieny S, Joiner K (1986) Evasion of the alternative complement pathway by metacyclic trypomastigotes of Tvypanosoma cruzi: dependence on the develop- mentally regulated synthesis of surface protein and N-linked carbohydrate. J Immunol 137:2961–2967PubMedGoogle Scholar
  3. 3.
    Pereira MEA (1983) A developmentally regulated neuraminidase activity in Tvypanosoma cruzi. Science 219:1444–1446PubMedCrossRefGoogle Scholar
  4. 4.
    Harth G, Haidaris CG, So M (1987) Purification and characterization of a neuraminidase, a developmentally regulated antigen in Tvypanosoma cruzi. In:Agabian N, Goodman H, Nogueira N (eds) Molecular strategies of parasitic invasion; UCLA Symp Mol Cell Biol, New Ser, vol 42. Liss, New York, pp 213–222Google Scholar
  5. 5.
    Varki A, Diaz S (1983) A neuraminidase from Streptooooous sanguis than can release O-acetylated sialic acids. J Biol Chem 258:12465–12471PubMedGoogle Scholar
  6. 6.
    Hunkapiller MW, Lujan E, Ostrander F, Hood LE (1983) Isolation of microgram quantities of proteins from polyacrylamide gels for amino acid sequence analysis. Meth Enzymol 91:227–236PubMedCrossRefGoogle Scholar
  7. 7.
    Konigsberg WH, Henderson L (1983) Removal of sodium dodecyl sulfate from proteins by ion-pair extraction. Meth Enzymol 91:254–259PubMedCrossRefGoogle Scholar
  8. 8.
    Towbin H, Staehelin T, Gordon J (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 76:4350–4354PubMedCrossRefGoogle Scholar
  9. 9.
    Navin TR, Roberto RR, Juranek DD, Limpakarnjanarat K, Mortenson EW, Clover JR, Yescott RE, Taclindo C, Steurer F, Allain D (1985) Human and sylvatic Tvypanosoma cruzi infection in California. Am J Publ Health 75:366–369CrossRefGoogle Scholar
  10. 10.
    McHardy N, Elphick JP (1978) Immunization of mice against infection with Tvypanosoma cruzi. Cross-immunization between five strains of the parasite using freeze-thawed vaccines containing epimastigotes of up to five strains. Int J Parasitol 8:25–31PubMedCrossRefGoogle Scholar
  11. 11.
    Tobie EJ (1961) Experimental transmission and biological comparison of strains of Tvypanosoma rangeli. Exp Parasitol 11:1–9PubMedCrossRefGoogle Scholar
  12. 12.
    Alexander J, Russell DG (1985) Parasite antigens, their role in protection, diagnosis and escape: The Leishmaniases. Curr Top Microbiol Immunol 120:43–67PubMedGoogle Scholar
  13. 13.
    Nickell SP, Gebremichael A, Hoff R, Boyer MH (1987) Isolation and functional characterization of murine T cell lines and clones specific for the protozoan parasite Tvypanosoma cruzi. J Immunol 138:914–921PubMedGoogle Scholar
  14. 14.
    Davis MM, Cohen DI, Nielsen EA, Steinmetz M, Paul WE, Hood L (1984) Cell-type- specific cDNA probes and the murine I region: the localization and orientation of Aαd. Proc Natl Acad Sci USA 81:2194–2198PubMedCrossRefGoogle Scholar
  15. 15.
    Norrander J, Kempe T, Messing J (1983) Construction of improved Ml3 vectors using oligodeoxynucleotide directed mutagenesis. Gene 26:101–106PubMedCrossRefGoogle Scholar
  16. 16.
    Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring HarborGoogle Scholar
  17. 17.
    Chen E, Seeburg PH (1985) Supercoil sequencing: a fast and simple method for sequencing plasmid DNA. DNA 4:165–170PubMedCrossRefGoogle Scholar
  18. 18.
    Wray W, Boulikas T, Wray V, Hancock R (1981) Silver staining of proteins in polyacrylamide gels. Anal Biochem 118:197–203PubMedCrossRefGoogle Scholar
  19. 19.
    Grace TDC (1962) Establishment of four strains of cells from insect tissues grown in vitro. Nature (London) 195:788–789CrossRefGoogle Scholar
  20. 20.
    Harth G, Haidaris CG, So M (1987) Neuraminidase from Trypanosoma cruzi: Analysis of enhanced expression of the enzyme in infectious forms. Proc Natl Acad Sci USA 84:8320–8324PubMedCrossRefGoogle Scholar
  21. 21.
    Warner TG, O’Brien JS (1979) Synthesis of 2’-(4-methylumbelliferyl)-a-D-N-ace- tylneuraminic acid and detection of skin fibroblast neuraminidase in normal humans and in sialidosis. Biochemistry 18:2783–2787PubMedCrossRefGoogle Scholar
  22. 22.
    Aminoff D (1961) Methods for the quantitative estimation of N-acetylneuraminic acid and their application to hydrolysates of sialomucoids. Biochem J 81:384– 392PubMedGoogle Scholar
  23. Haidaris CG, Harth G, So M (1987) Isolation and characterization of stage specifically expressed genes in Trypanosoma cruzí. (in preparation)Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1987

Authors and Affiliations

  • G. Harth
  • C. G. Haidaris
  • M. So
    • 1
  1. 1.Department of Molecular Biology MB-4Research Institute of Scripps ClinicLa JollaUSA

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