Electron Histochemical Localization of Calcium in CA1 of Hippocampal Slices Following High Frequency Stimulation: Effects of Excitatory Amino Acid Antagonists
Experiments were performed to localize calcium in CA1 of hippocampal slices (i) under control condition, (ii) after generation of LTP, (iii) after blockade of LTP by APV, and (iiii) after blockade of synaptic transmission by GDEE. The physiological experiments were followed by immersion fixation in modified Winborn fixative and the pattern of Ca-containing deposits was investigated. The dendritic, deposits were restricted to a sublamina of str.radiatum. The highest density of dendritic deposits was found 280–350 μm distal to the str.pyramidale. The location of this sublamina correlated with the position of the stimulating electrode.
KeywordsHippocampal Slice Stimulate Electrode High Frequency Stimulation Tetanic Stimulation Pyramidal Cell Layer
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