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Electron Histochemical Localization of Calcium in CA1 of Hippocampal Slices Following High Frequency Stimulation: Effects of Excitatory Amino Acid Antagonists

  • L. Siklos
  • U. Kuhnt
  • F. Joo
  • A. Mihaly
Conference paper

Summary

Experiments were performed to localize calcium in CA1 of hippocampal slices (i) under control condition, (ii) after generation of LTP, (iii) after blockade of LTP by APV, and (iiii) after blockade of synaptic transmission by GDEE. The physiological experiments were followed by immersion fixation in modified Winborn fixative and the pattern of Ca-containing deposits was investigated. The dendritic, deposits were restricted to a sublamina of str.radiatum. The highest density of dendritic deposits was found 280–350 μm distal to the str.pyramidale. The location of this sublamina correlated with the position of the stimulating electrode.

Keywords

Hippocampal Slice Stimulate Electrode High Frequency Stimulation Tetanic Stimulation Pyramidal Cell Layer 
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References

  1. 1.
    U.Kuhnt, A.Mihaly, F.Joo: Increased binding of calcium in the hippocampal slice during long-term potentiation. Neuroscience Lett., 53 (1985) 149–154CrossRefGoogle Scholar
  2. 2.
    U.Kuhnt, A.Mihaly, F.Joo, L.Siklos: Localization of calcium in long-term potentiated CA1 region of the hippocampal slice: a combined electrophysiological and histochemical study. SubmittedGoogle Scholar
  3. 3.
    A.Mihaly, U.Kuhnt, F.Joo, L.Siklos: Semi-quantitative evaluation of histochemically detectable calcium binding in mammalian brain slices. J. Neurosc. Meth., 20 (1987), 283–293CrossRefGoogle Scholar
  4. 4.
    L.Siklos, F.Joo, U.Kuhnt: Method for visualizing the spatial distribution of histochemical precipitates of large areas at the EM-level. SubmittedGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1988

Authors and Affiliations

  • L. Siklos
    • 1
    • 3
  • U. Kuhnt
    • 2
    • 3
  • F. Joo
    • 1
    • 3
  • A. Mihaly
    • 1
    • 3
  1. 1.Institute of BiophysicsBiological Research CenterSzegedHungary
  2. 2.Max-Planck-lnstitut für Biophysikalische ChemieGöttingenFederal Republic of Germany
  3. 3.Department of AnatomyUniversity Medical SchoolSzegedHungary

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