Electron Histochemical Localization of Calcium in CA1 of Hippocampal Slices Following High Frequency Stimulation: Effects of Excitatory Amino Acid Antagonists

Siklos, L.; Kuhnt, U.; Joo, F.; Mihaly, A.

doi:10.1007/978-3-642-73202-7_15

L. Siklos^3,5,
U. Kuhnt^4,5,
F. Joo^3,5 &
…
A. Mihaly^3,5

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Summary

Experiments were performed to localize calcium in CA1 of hippocampal slices (i) under control condition, (ii) after generation of LTP, (iii) after blockade of LTP by APV, and (iiii) after blockade of synaptic transmission by GDEE. The physiological experiments were followed by immersion fixation in modified Winborn fixative and the pattern of Ca-containing deposits was investigated. The dendritic, deposits were restricted to a sublamina of str.radiatum. The highest density of dendritic deposits was found 280–350 μm distal to the str.pyramidale. The location of this sublamina correlated with the position of the stimulating electrode.

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References

U.Kuhnt, A.Mihaly, F.Joo: Increased binding of calcium in the hippocampal slice during long-term potentiation. Neuroscience Lett., 53 (1985) 149–154
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U.Kuhnt, A.Mihaly, F.Joo, L.Siklos: Localization of calcium in long-term potentiated CA1 region of the hippocampal slice: a combined electrophysiological and histochemical study. Submitted
Google Scholar
A.Mihaly, U.Kuhnt, F.Joo, L.Siklos: Semi-quantitative evaluation of histochemically detectable calcium binding in mammalian brain slices. J. Neurosc. Meth., 20 (1987), 283–293
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L.Siklos, F.Joo, U.Kuhnt: Method for visualizing the spatial distribution of histochemical precipitates of large areas at the EM-level. Submitted
Google Scholar

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Authors and Affiliations

Institute of Biophysics, Biological Research Center, 6701, Szeged, Hungary
L. Siklos, F. Joo & A. Mihaly
Max-Planck-lnstitut für Biophysikalische Chemie, 3400, Göttingen, Federal Republic of Germany
U. Kuhnt
Department of Anatomy, University Medical School, 6701, Szeged, Hungary
L. Siklos, U. Kuhnt, F. Joo & A. Mihaly

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L. Siklos
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U. Kuhnt
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F. Joo
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A. Mihaly
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Editors and Affiliations

II. Physiologisches Institut, Johannes Gutenberg-Universität, Saarstrasse 21, 6500, Mainz, Germany
Helmut L. Haas M.D.
Department of Neurosciences MO24, University of California at San Diego, 92093, La Jolla, CA, USA
György Buzsàki M.D.

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Siklos, L., Kuhnt, U., Joo, F., Mihaly, A. (1988). Electron Histochemical Localization of Calcium in CA1 of Hippocampal Slices Following High Frequency Stimulation: Effects of Excitatory Amino Acid Antagonists. In: Haas, H.L., Buzsàki, G. (eds) Synaptic Plasticity in the Hippocampus. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-73202-7_15

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DOI: https://doi.org/10.1007/978-3-642-73202-7_15
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-73204-1
Online ISBN: 978-3-642-73202-7
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