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A Genetic Analysis of Pullulanase Export from Klebsiella Aerogenes

  • M. G. Kornacker
  • A. Boyd
  • G. S. Plastow
Conference paper
Part of the NATO ASI Series book series (volume 16)

Abstract

The enzyme pullulanase (EC 3.2.1.41) is produced by various genera of Gram-positive and Gram-negative bacteria. This enzyme is defined by its ability to hydrolyze α(1–6) bonds of α(1–4), α(1–6) glucans. Pullulanases of approximately 140 kd (Eisele et al. 1972) are produced and secreted by strains of the Gram-negative bacterium Klebsiella aerogenes. The amounts synthesized and proportions secreted are strain-dependent. In this report we summarize data obtained from the analysis of Klebsiella aerogenes strain K21. This strain secretes 50% of pullulanase made into the culture medium. The remaining 50% is located at the cell surface.

Keywords

HindIII Fragment BglII Fragment Maltose Utilisation Lipoprotein Nature Signal Peptidase Cleavage Site 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1988

Authors and Affiliations

  • M. G. Kornacker
    • 1
  • A. Boyd
    • 2
  • G. S. Plastow
    • 1
  1. 1.The Leicester BiocentreUniversity of LeicesterLeicesterGreat Britain
  2. 2.Dept. of Biochemistry, Medical SchoolUniversity of EdinburghEdinburghUK

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