A Genetic Analysis of Pullulanase Export from Klebsiella Aerogenes
The enzyme pullulanase (EC 220.127.116.11) is produced by various genera of Gram-positive and Gram-negative bacteria. This enzyme is defined by its ability to hydrolyze α(1–6) bonds of α(1–4), α(1–6) glucans. Pullulanases of approximately 140 kd (Eisele et al. 1972) are produced and secreted by strains of the Gram-negative bacterium Klebsiella aerogenes. The amounts synthesized and proportions secreted are strain-dependent. In this report we summarize data obtained from the analysis of Klebsiella aerogenes strain K21. This strain secretes 50% of pullulanase made into the culture medium. The remaining 50% is located at the cell surface.
KeywordsHindIII Fragment BglII Fragment Maltose Utilisation Lipoprotein Nature Signal Peptidase Cleavage Site
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