Membrane Structure and Dynamics by NMR. Part 2: The Action of Polyene Antibiotics and Toxins on Biological Membranes

  • Erick J. Dufourc
Conference paper
Part of the NATO ASI Series book series (volume 16)


Part 1 of this contribution dealt with properties of the membrane itself and has shown that relatively small modifications in the lipid structure or in the sterol structure can lead to very important changes in membrane dynamics.


Acyl Chain Quadrupolar Splitting Powder Pattern Label Position Pure Lipid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Abragam A (1961) Principles of nuclear magnetism. Oxford University Press LondonGoogle Scholar
  2. Davis JH (1983) The description of membrane lipid conformation order and dynamics by 2H-NMR. Biochim Biophys Acta 737: 117–171PubMedGoogle Scholar
  3. De Kruijff B, Demel RA (1974) Polyene antibiotic sterol interactions in membranes of Acholeplasma laidlawii cells and lecithin liposomes. Part 3: molecular structure of the polyene antibiotic cholesterol complexes. Biochim Biophys Acta 339: 57–70PubMedCrossRefGoogle Scholar
  4. De Kruijff B, Gerritsen WJ, Oerlemans A, Demel R, Van Deenen LLM (1974a) Polyene antibiotic sterol interactions in membranes of Acholeplasma laidlawii cells and lecithin liposomes. Part 1: specificity of the membrane permeability changes induced by the polyene antibiotics. Biochim Biophys Acta 339: 30–43PubMedCrossRefGoogle Scholar
  5. De Kruijff B, Gerritsen WJ, Oerlemans A, Demel R, Van Deenen LLM (1974b) Polyene antibiotic sterol interactions in membranes of Acholeplasma laidlawii cells and lecithin liposomes. Part 2: temperature dependence of the polyene antibiotic sterol complex formation. Biochim Biophys Acta 339: 44–56PubMedCrossRefGoogle Scholar
  6. Dufourc EJ (1986) La résonance magnétique nucléaire du deutérium et du phosphore dans les milieux organisés. Théorie et applications à l’étude de l’action de la melittine et de la delta-lysine sur les membranes biologiques. University of Bordeaux I BordeauxGoogle Scholar
  7. Dufourc EJ, Smith ICP (1985) 2H-NMR evidence for antibiotic- induced cholesterol immobilization in biological model membranes. Biochemistry 24: 2420–2424PubMedCrossRefGoogle Scholar
  8. Dufourc EJ, Smith ICP (1986) A detailed analysis of the motions of cholesterol in biological membranes by 2H-NMR relaxation. Chem Phys Lipids 41: 123–135PubMedCrossRefGoogle Scholar
  9. Dufourc EJ, Smith ICP, Jarrell HC (1984a) Interaction of Amphotericin B with membrane lipids as viewed by 2H-NMR. Biochim Biophys Acta 778: 435–442PubMedCrossRefGoogle Scholar
  10. Dufourc EJ, Parish EJ, Chitrakorn S, Smith ICP (1984b) Structural and dynamical details of cholesterol-1ipid interaction as revealed by deuterium NMR. Biochemistry 23: 6062–6071CrossRefGoogle Scholar
  11. Dufourc EJ, Smith ICP, Jarrell HC (1984c) Amphotericin B and model membranes: the effect of Amphotericin B on cholesterol-containing systems as viewed by 2H-NMR. Biochim Biophys Acta 776: 317–329PubMedCrossRefGoogle Scholar
  12. Dufourc EJ, Smith ICP, Dufourcq J (1986a) Molecular details of melittin-induced lysis of phospholipid membranes as revealed by deuterium and phosphorus NMR. Biochemistry 25: 6448–6455PubMedCrossRefGoogle Scholar
  13. Dufourc EJ, Faucon JF, Fourche G, Dufourcq J, Gulik-Krzywicki T, Le Maire M (1986b) Reversible disc-to-vesicle transition of melittin-DPPC complexes triggered by the phospholipid acyl chain melting. FEBS Letters 201: 205–209CrossRefGoogle Scholar
  14. Dufourcq J, Faucon JF, Fourche G, Dasseux JL, Le Maire M, Guiik-Krzywicki T (1986) Morphological changes of phosphatidylcholine bilayers induced by melittin: vesicularization, fusion, discoidal particles. Biochim Biophys Acta 859: 33–48PubMedCrossRefGoogle Scholar
  15. Gottlieb D, Carter HE, Sloneker JH, Ammon A (1958) Protection of fungi against polyene antibiotic by sterols. Science 128: 361PubMedCrossRefGoogle Scholar
  16. Haberman E (1972) Bee and wasp venoms. Science (Washington DC) 177: 314–222CrossRefGoogle Scholar
  17. Hazen EL, Brown R (1951) Fungicidin, an antibiotic produced by soil actinomycete. Proc Soc Exp Biol Med 76: 93–97PubMedGoogle Scholar
  18. Hegner D (1968) The effect of melittin hematal on isolated lysosomal granules and polymorphonuclear leukocytes in vitro bovine. Naunyn-Schmiedebergs Arch Pharmakol Exp Pathol 261: 118–127Google Scholar
  19. Kinsky SC (1961a) Effect of polyene antibiotics on permeability in Neurospora crassa. Biochem Biophys Res Commun 4: 353–357PubMedCrossRefGoogle Scholar
  20. Kinsky SC (1961b) Alterations in the permeability of Neurospora crassa due to polyene antibiotics. J Bacteriol 82: 889–897PubMedGoogle Scholar
  21. Mollay C, Kreil G, Berger H (1976) Action of phospholipase, on the cytoplasmic membrane of Escheri chia coli stimulation by melittin. Biochim Biophys Acta 426: 317–324PubMedCrossRefGoogle Scholar
  22. Norman AW, Spielvogel AM, Wong RG (1976) Polyene antibiotic sterol interaction. Adv Lipid Res 14: 127–170PubMedGoogle Scholar
  23. Olson FC, Munjal D, Malriya AN (1974) Structural and respiratory effects of melittin apis-mellifera on rat liver mitochondria. Toxicon 12: 419–425PubMedCrossRefGoogle Scholar
  24. Seelig J (1977) Deuterium magnetic resonance: theory and application to lipid membranes. Quart Rev Biophys 10: 353–418CrossRefGoogle Scholar
  25. Sessa G, Freer JH, Colacicco G, Weismann G (1969) Interaction of a lytic polypeptide melittin with lipid membrane systems. J Biol Chem 244: 3575–3582PubMedGoogle Scholar
  26. Taylor MG, Akiyama T, Smith ICP (1981) The molecular dynamics of cholesterol in bilayer membranes: a deuterium NMR study. Chem Phys Lipids 29: 327–339CrossRefGoogle Scholar
  27. Weissmann G, Hirschlorn R, Kreakaver K (1969) Effect of melittin upon cellular and lysosomal membranes. Biochem Pharmacol 18: 1771–1775PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1988

Authors and Affiliations

  • Erick J. Dufourc
    • 1
  1. 1.C.R. Paul Pascal/CNRSDomaine UniversitaireTalence CedexFrance

Personalised recommendations