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Occurrence of a Syringomycin — High Molecular Weight Complex in Pseudomonas Syringae PV. Syringae

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Part of the NATO ASI Series book series (ASIH,volume 27)

Abstract

Selected pathogenic strains of Pseudomonas syringae pv. syri ngae van Hall produce in culture syringomycin (SR), a low-molecular-weight, peptide-containing Phytotoxin which is reported to play a role in diseases caused by this bacterium. In addition, a large number of bacteria and fungi, including Geotrichum candi dum, are sensitive to SR. The toxin traditionally has been isolated by treating P. syringae pv. syri ngae cultures with 50% acetone at pH 2 (cells and filtrate combined), removing the precipitate by centrifugation, reducing the volume about 20-fold in vacuo and then extracting with n-butanol. The active extract is purified to homogeneity by cation-exchange chromatography (Gross and DeVay, 1977).

Keywords

  • Culture Filtrate
  • Ammonium Sulphate
  • Ammonium Sulphate Precipitate
  • High Molecular Weight Fraction
  • Geotrichum Candidum

These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  • Gross DC, DeVay JE (1977) Production and purification of syringomycin, a Phytotoxin produced by Pseudomonas syringae. Physiol Plant Pathol 11: 13–28

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  • Surico G, Iacobellis NS (1977) Confronto di alcuni indici di virulenza di isolati di Pseudomonas syringae van Hall da fagiolo. Phytopath medit 16:79–86

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© 1989 Springer-Verlag Berlin Heidelberg

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Iacobellis, N.S., Lavermicocca, P., Surico, G., Durbin, R.D. (1989). Occurrence of a Syringomycin — High Molecular Weight Complex in Pseudomonas Syringae PV. Syringae . In: Graniti, A., Durbin, R.D., Ballio, A. (eds) Phytotoxins and Plant Pathogenesis. NATO ASI Series, vol 27. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-73178-5_50

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  • DOI: https://doi.org/10.1007/978-3-642-73178-5_50

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-73180-8

  • Online ISBN: 978-3-642-73178-5

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