Occurrence of a Syringomycin — High Molecular Weight Complex in Pseudomonas Syringae PV. Syringae

  • N. S. Iacobellis
  • P. Lavermicocca
  • G. Surico
  • R. D. Durbin
Conference paper
Part of the NATO ASI Series book series (volume 27)

Abstract

Selected pathogenic strains of Pseudomonas syringae pv. syri ngae van Hall produce in culture syringomycin (SR), a low-molecular-weight, peptide-containing Phytotoxin which is reported to play a role in diseases caused by this bacterium. In addition, a large number of bacteria and fungi, including Geotrichum candi dum, are sensitive to SR. The toxin traditionally has been isolated by treating P. syringae pv. syri ngae cultures with 50% acetone at pH 2 (cells and filtrate combined), removing the precipitate by centrifugation, reducing the volume about 20-fold in vacuo and then extracting with n-butanol. The active extract is purified to homogeneity by cation-exchange chromatography (Gross and DeVay, 1977).

Keywords

Toxicity HPLC Acetone Carbohydrate Acetonitrile 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Gross DC, DeVay JE (1977) Production and purification of syringomycin, a Phytotoxin produced by Pseudomonas syringae. Physiol Plant Pathol 11: 13–28Google Scholar
  2. Surico G, Iacobellis NS (1977) Confronto di alcuni indici di virulenza di isolati di Pseudomonas syringae van Hall da fagiolo. Phytopath medit 16:79–86Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1989

Authors and Affiliations

  • N. S. Iacobellis
    • 1
  • P. Lavermicocca
    • 1
  • G. Surico
    • 2
  • R. D. Durbin
    • 3
  1. 1.Istituto tossine e micotossine da parassiti vegetali del C.N.R.BariItaly
  2. 2.Istituto di Patologia e Zoologia Forestale e AgrariaUniversità di FirenzeItaly
  3. 3.ARS, USDA and Department of Plant PathologyUniversity of WisconsinMadisonUSA

Personalised recommendations