Occurrence of a Syringomycin — High Molecular Weight Complex in Pseudomonas Syringae PV. Syringae
Selected pathogenic strains of Pseudomonas syringae pv. syri ngae van Hall produce in culture syringomycin (SR), a low-molecular-weight, peptide-containing Phytotoxin which is reported to play a role in diseases caused by this bacterium. In addition, a large number of bacteria and fungi, including Geotrichum candi dum, are sensitive to SR. The toxin traditionally has been isolated by treating P. syringae pv. syri ngae cultures with 50% acetone at pH 2 (cells and filtrate combined), removing the precipitate by centrifugation, reducing the volume about 20-fold in vacuo and then extracting with n-butanol. The active extract is purified to homogeneity by cation-exchange chromatography (Gross and DeVay, 1977).
KeywordsCulture Filtrate Ammonium Sulphate Ammonium Sulphate Precipitate High Molecular Weight Fraction Geotrichum Candidum
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