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A Molecular Strategy for Isolation of the TOXl Allele from Cochliobolus Heterostrophus

  • B. G. Turgeon
  • L. Ciuffetti
  • W. Schafer
  • O. C. Yoder
Conference paper
Part of the NATO ASI Series book series (volume 27)

Abstract

The fungal maize pathogen Cochliobolus heterostrophus (anamorph: Helminthosporium maydis) exists as two pathotypes: race T, which produces T-toxin and race O, which does not. All race T isolates are highly virulent on maize with Texas male sterile cytoplasm; race O isolates are weakly virulent. On maize lines lacking T-cytoplasm, the virulence of races T and O is comparable. Crosses between isolates of races T and O have demonstrated that T-toxin production is determined by a single locus, Tox1. TOX1 progeny produce T-toxin and are highly virulent on T-cytoplasm maize; tox1 progeny do not produce T-toxin and are weakly virulent. Dominance of the TOX1 allele has been established by hetero-karyon analysis (Leach et al, 1982).

Keywords

Aspergillus Nidulans Maize Line Molecular Strategy Haploid Genome Size Coli Genome 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. Clarke L, Carbon J (1976) A colony bank containing synthetic ColE1 hybrid Plasmids representative of the entire E. coli genome. Cell 9:91–99Google Scholar
  2. Dewey RE, Seidow JN, Timothy DH, Levings III CS (1987) A 13 kilodalton maize mitochondrial protein in E. coli confers sensitivity to Bipolaris maydis toxin. Science 239:293–295CrossRefGoogle Scholar
  3. Leach J, Tegtmeier J, Daly JM, Yoder OC (1982) Dominance at the Tox1 locus controlling T-toxin production by Cochliobolus heterostrophus. Physiol Plant Pathol 21:327–333CrossRefGoogle Scholar
  4. Turgeon BG, Garber RC, Yoder OC (1985) Transformation of the fungal maize pathogen Cochliobolus heterostrophus using the Aspergillus nidulans amdS gene. Mol Gen Genet 201:450–453CrossRefGoogle Scholar
  5. Turgeon BG, Garber RC, Yoder OC (1987) Development of a fungal transformation system based on selection of sequences with promoter activity. Mol Cell Biol 7:3297–3305.PubMedGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1989

Authors and Affiliations

  • B. G. Turgeon
    • 1
  • L. Ciuffetti
    • 1
  • W. Schafer
    • 1
  • O. C. Yoder
    • 1
  1. 1.Department of Plant PathologyCornell UniversityIthacaUSA

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