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Potassium Channels in Rat Brain Synaptosomes: Pharmacology and Toxicology

  • Mordecai P. Blaustein
  • Dieter K. Bartschat
  • Christina G. Benishin
  • William E. Brown
  • Kathryn A. Colby
  • Bruce K. Krueger
  • Mary J. Schneider
  • Roger G. Sorensen
Part of the NATO ASI Series book series (volume 21)

Abstract

Potassium channels appear to be the most diverse group of ion channels in biological systems (Hille, 1984; Yellen, 1987). Neuronal K channels play key roles in the control of membrane potential, action potential repolarization, repetitive firing, and higher functions such as learning and memory. However, relatively little is known about the properties of K channels in presynaptic nerve terminals because these channels are difficult to study with traditional electrophysiological methods. This is a significant gap in our knowledge because these nerve terminal K channels may play a critical role in the control of synaptic transmission.

Keywords

Synaptic Membrane Scorpion Toxin Presynaptic Nerve Terminal Delayed Rectifier Snake Toxin 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Albuquerque EX, Aguayo LG, Warnick JE, Weinstein H, Glick SD, Maayani S, Ickowicz R and Blaustein MP (1981) The behavioral effects of phencyclidines may be due to their blockade of potassium channels. Proc Natl Acad Sei USA 78: 7792–7796CrossRefGoogle Scholar
  2. Amando-Hardy M, Ellory JC, Ferreira MG, Fleminger S and Lew VL (1975) Inhibition of the calcium-induced increase in the potassium permeability of human red blood cells by quinine. J Physiol (Lond) 250: 32–33 PGoogle Scholar
  3. Bartschat DK and Blaustein MP (1985a) Potassium channels in isolated presynaptic nerve terminals from rat brain. J Physiol (Lond) 361: 419– 440Google Scholar
  4. Bartschat DK and Blaustein MP (1985b) Calcium–activated potassium channels in presynaptic nerve terminals from rat brain. J Physiol (Lond) 361: 441–457Google Scholar
  5. Bartschat DK and Blaustein MP (1986) Phencyclidine in low doses selectively blocks a presynaptic voltage-regulated potassium channel in rat brain. Proc Natl Acad Sei USA 83: 189–192CrossRefGoogle Scholar
  6. Bartschat DK and Blaustein MP (1988) Psychotomimetic sigma ligands, dexoxadrol and phencyclidine block the same presynaptic potassium channel in rat brain. J Physiol (Lond) in pressGoogle Scholar
  7. Benishin CG, Sorensen RG, Krueger BK and Blaustein MP (1987) Four toxin components of green mamba (Dendroaspis angusticeps) venom with different specificities for voltage–gated K channels in rat brain synaptosomes. Fed Proc 46: 504Google Scholar
  8. Blatz AL and Magleby KL (1986) Single apamin-blocked Ca-activated K+ channels of small conductance in cultured rat skeletal muscle. Nature 323: 718–720PubMedCrossRefGoogle Scholar
  9. Carbone E, Prestipino G, Spadavecchia L, Franciolini F and Possani LD (1987) Blocking of the squid axon K+ channel by noxiustoxin: a toxin from the venom of the scorpion Centruroides noxius. Pflugers Arch 408: 423–431PubMedCrossRefGoogle Scholar
  10. Castellucci VF, Kandel ER, Schwartz JH, Wilson FD, Nairn AC and Greengard P (1980) Intracellular injection of the catalytic subunit of cyclic AMP-dependent protein kinase simulates facilitation of transmitter release underlying behavioral sensitization in Aplysia. Proc Natl Acad Sci USA 77: 7492–7496PubMedCrossRefGoogle Scholar
  11. Cone EJ, McQuinn RL and Shannon HE (1984) Structure–activity relationship studies of phencyclidine derivatives in rats. J Pharmacol Exp Therap 228: 147–153Google Scholar
  12. Conn PM, Ganong BR, Ebeling J, Staley D, Neidel JE and Bell RM (1985) Diacylglycerols release LH: Structure-activity relations reveal a role for protein kinase C. Biochem Biophys Res Comm 126: 532–539PubMedCrossRefGoogle Scholar
  13. Dinnan TG, Crunelli V and Kelly JS (1987) Neuroleptics decrease calcium-activated potassium conductance in hippocampal pyramidal cells. Brain Research 407: 159–162CrossRefGoogle Scholar
  14. Dolly JO, Halliwell JV, Black JD, Williams RS, Pelchen-Matthews A, Breeze AL, Mehraban F, Othman IB and Black AR (1984) Botulinum neurotoxin and dendrotoxin as probes for studies on transmitter release. J Physiol (Paris) 74: 280–303Google Scholar
  15. Domino EF (ed) (1981) PCP (Phencyclidine): Historical and Current Perspectives. NPP Books, Ann Arbor, MI.Google Scholar
  16. Dufton M.J. Protease inhibitors and dendrotoxins. Sequence classification, structural prediction and structure/activity. Eur J Biochem 153: 647–654 (1985).PubMedCrossRefGoogle Scholar
  17. Farley J and Auerbach S (1986) Protein kinase C activation induces conductance changes in Hermissenda photoreceptors like those seen in associative learning. Nature 319: 220–223PubMedCrossRefGoogle Scholar
  18. Hajos F (1975) An improved method for the preparation of synaptosomal fractions in high purity. Brain Res 93: 485–489PubMedCrossRefGoogle Scholar
  19. Halliwell JV, Othman IB, Pelchan-Matthews A and Dolly JO (1986) Central action of dendrotoxin: selective reduction of a transient K conductance in hippocampus and binding to localized acceptors. Proc Natl Acad Sci USA 83: 493–497PubMedCrossRefGoogle Scholar
  20. Hampton RY, Mdzihradsky F Woods JH and Dahlstrom PJ (1982) Stereospecific binding of phencyclidine in brain membranes. Life Sci 30: 2147–2154PubMedCrossRefGoogle Scholar
  21. Hannun YA, Loomis CR, Merrill AH Jr. and Bell RM (1986) Sphingosine inhibition of protein kinase C activity and of phorbol dibutyrate binding in vitro and in human platelets. J Biol Chem 261: 12604–12609PubMedGoogle Scholar
  22. Harvey AL and Karlsson E (1980) Dendrotoxin from the venom of the green mamba, Dendroaspis angusticeps. A neurotoxin that enhances acetylcholine release at neuromuscular junctions. Naunyn-Schmeideburg’s Arch Pharmacol 312: 1–6CrossRefGoogle Scholar
  23. Harvey AL and Karlsson E (1982) Protease inhibitor homologues from mamba venoms: facilitation of acetylcholine release and interactions with prejunctional blocking toxins. Br J Pharmacol 77: 153–161PubMedGoogle Scholar
  24. Hille B (1984) Ionic channels of excitable membranes. Sinauer Associates, Inc. Sunderland, MAGoogle Scholar
  25. Hugues M, Romey G, Duval D, Vincent JP and Lazdunski M (1982) Apamin as a selective blocker of the calcium dependent potassium channel in neuroblastoma cells: voltage clamp and biochemical characterization of the toxin receptor. Proc Natl Acad Sci USA 79: 1308–1312PubMedCrossRefGoogle Scholar
  26. Joubert FJ and Taljaard N (1980) The amino acid sequence of two proteinase inhibitor homologues from Dendroaspis angusticeps venom. Hoppe-Seyler’s Z. Physiol. Chem 361: 661–674CrossRefGoogle Scholar
  27. Krueger BK and Blaustein MP (1980) Sodium channels in presynaptic nerve terminals. Regulation by neurotoxins. J Gen Physiol 76: 287–313PubMedCrossRefGoogle Scholar
  28. Krueger BK, Ratzlaff RW, Strichartz GR and Blaustein MP (1979) Saxitoxin binding to synaptosomes, membranes, and solubilized binding sites from rat brain. J Membrane Biol 50: 287–310CrossRefGoogle Scholar
  29. Levitan IB (1985) Phosphorylation of ion channels. J Membrane Biol 87: 177–190CrossRefGoogle Scholar
  30. Malenka RC, Madison DV and Nicholl RA (1986) Potentiation of synaptic transmission in the hippocampus by phorbol esters. Nature 321: 695– 697Google Scholar
  31. McCann JD and Welsh MJ (1987) Neuroleptics antagonize a calcium-activated potassium channel in airway smooth muscle. J Gen Physiol 89: 339–352PubMedCrossRefGoogle Scholar
  32. Mehraban F, Breeze AL and Dolly JO (1984) Identification by cross-linking of a neuronal acceptor protein for dendrotoxion, a convulsant peptide. FEBS Lett. 174: 116–122PubMedCrossRefGoogle Scholar
  33. Miller C, Moczydlowski E, Latorre R and Phillips M (1985) Charybdotoxin, a protein inhibitor of single Ca2+-activated K+ channels from mammalian skeletal muscle. Nature (Lond) 313: 316–318CrossRefGoogle Scholar
  34. Nachshen DA (1985) The early time course of potassium–stimulated calcium influx into presynaptic terminals from rat brains. J Physiol (Lond) 361: 251–268Google Scholar
  35. Nachshen DA and Blaustein MP (1980) Some properties of potassium-stimulated calcium influx in presynaptic nerve endings. J Gen Physiol 76: 709–728PubMedCrossRefGoogle Scholar
  36. Nachshen DA and Blaustein MP (1982) Influx of calcium, strontium and barium in presynaptic nerve endings. J Gen Physiol 79: 1065–1087PubMedCrossRefGoogle Scholar
  37. Possani LD (1982) The primary structure of noxiustoxin: a K+ channel blocking peptide, purified from the venom of the scorpion Centruroides noxius Hoffmann. Carlsberg Res Commun 47: 285–289CrossRefGoogle Scholar
  38. Possani LD (1984) Structure of scorpion toxins. In: Tu AT (ed). Handbook of natural toxins. Vol. 2. Insect poisons, allergens and other invertebrate venoms. Marcel Dekker 1984 New York: 513–550Google Scholar
  39. Ragowski MA (1985) The A-current: How ubiquitous a feature of excitable cells is it? Trends Neurosci 8: 214–219CrossRefGoogle Scholar
  40. Romey G and Lazdunski M (1984) The coexistence in rat muscle of two distinct classes of Ca2+ -dependent K+ channels with different pharmacological properties and different physiological functions. Biochem Biophys Research Commun 118: 669–674CrossRefGoogle Scholar
  41. Salacinski PRP, McLean C, Sykes JEC, Clement-Jones W and Lowry PJ (1981) Iodinationof proteins, glycoproteins, and peptides using a solid-phase oxidizing agent, 1,3,4,6 –tetrachloro-3a,6a-diphenyl glycoluril (iodogen). Analyt Biochem 117: 136–146PubMedCrossRefGoogle Scholar
  42. Smith C, Phillips M and Miller C (1986) Purification of charybdotoxin, a specific inhibitor of the high-conductance Ca2+-activated K+ channel. J Biol Chem 261: 14607–14613PubMedGoogle Scholar
  43. Sorensen RG, and Blaustein MP (1986) m-Azido-Phencyclidine covalently labels the rat brain PCP receptor, a putative K channel. J Neurosci 6: 3676–3681Google Scholar
  44. Sorensen RG and Blaustein MP (1987) The rat brain phencyclidine (PCP) receptor. A putative K channel. Biochem Pharmacol, in press.Google Scholar
  45. Takai Y, Kishimoto A, Iwasa Y, Kawahara Y, Mori T and Nishizuka Y (1979) Calcium-dependent activation of a multifunctional protein kinase by membrane phospholipids. J Biol Chem 254: 3692–3695PubMedGoogle Scholar
  46. Tamkun MM and Catterall WA (1981) Ion flux studies of voltage-sensitive sodium channels in synaptic nerve-ending particles. Molec Pharmacol 19: 78–86Google Scholar
  47. Williams JT, Egan TM and North RA (1982) Enkephalin opens potassium channels on mammalian central neurones. Nature 299: 74–77PubMedCrossRefGoogle Scholar
  48. Yellen G (1987) Permeation in potassium channels: implications for channel structure. Ann Rev Biophys Biophys Chem 16: 227–246CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1988

Authors and Affiliations

  • Mordecai P. Blaustein
    • 1
  • Dieter K. Bartschat
    • 1
  • Christina G. Benishin
    • 1
  • William E. Brown
    • 2
  • Kathryn A. Colby
    • 1
  • Bruce K. Krueger
    • 1
  • Mary J. Schneider
    • 1
  • Roger G. Sorensen
    • 1
  1. 1.Department of PhysiologyUniversity of Maryland School of MedicineBaltimoreUSA
  2. 2.Department of Biological SciencesCarnegie-Mellon UniversityPittsburghUSA

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