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Mechanisms of Adaptation to Salinity in Cultured Glycophyte Cells

  • M. L. Binzel
  • F. D. Hess
  • R. A. Bressan
  • P. M. Hasegawa
Part of the NATO ASI Series book series (volume 19)

Summary

Cultured cells of Nicotiana tabacum var Wisconsin 38 can be adapted to grow in levels of NaCl similar to those tolerated by many halophytes. Adaptation of these cells to high levels of salinity is associated with reduced cell expansion even though turgor is maintained, a result similar to that commonly reported for whole plants exposed to salinity and/or drought. Glycophytic cells adapted to salinity apparently utilize many of the same biochemical and physiological processes to deal with salinity as do halophytes. Na+ and Cl- are the principal solutes contributing to the extensive osmotic adjustment these cells undergo in response to salinity, although organic solutes, particularly proline, accumulate as well. Adapted cells accumulate less Na+ than unadapted cells when grown in comparable levels of NaCl, suggesting that the ability to regulate ion accumulation may be an important component of the salinity tolerance of these cells. The majority of Na+ and Cl- accumulated by the cells is compartmentalized in the vacuole, such that cytosolic levels of these ions remain near 100 mM at external NaCl concentrations of 428 mM. Current research is directed at examining changes in membrane transport properties associated with salinity in order to assess their contribution to the ability of the cells to tolerate salt.

Keywords

Salt Tolerance Salinity Tolerance Tonoplast Vesicle Isobutyl Ester Unadapted Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1989

Authors and Affiliations

  • M. L. Binzel
    • 1
  • F. D. Hess
    • 1
    • 2
  • R. A. Bressan
    • 1
  • P. M. Hasegawa
    • 1
  1. 1.Center for Plant Environmental Stress Physiology, Department of HorticulturePurdue UniversityW. LafayetteUSA
  2. 2.Zoecon Research Institute, Sandoz Crop Protection InstitutePalo AltoUSA

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