Participation of Regulatory G-Proteins and Protein Kinase C in the Modulation of Transmitter Release in Hippocampus

  • G. Hertting
  • S. Wurster
  • P. Gebicke-Härter
  • C. Allgaier
Part of the NATO ASI Series book series (volume 19)


1.) Regulatory G-proteins participate in the inhibition of noradrenaline (NA) and acetylcholine (ACh) release following activation of the respective autoreceptors (α2, M2). Isletactivating protein (IAP) and N-ethylmaleimide (NEM) increased the electrically evoked release of [3 H]NA and [3 H]ACh from hippocampal slices and reduced inhibition of release by the autoreceptor agonists clonidine and carbachol as well as its facilitation by the antagonists yohimbine and atropine. In synaptosomes prepared from rabbit hippocampus two polypeptides with molecular weights corresponding to those of Gi α and Go α a were [32P]ADP-ribosylated by IAP in the presence of [3 2P]NAD+. Pretreatment of synaptosomes with NEM reduced the subsequent [3 2P]ADP-ribosylation by IAP in a concentration-dependent manner.


Hippocampal Slice Phorbol Ester Transmitter Release Noradrenaline Release Adenosine Receptor Agonist 
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Copyright information

© Springer-Verlag Berlin Heidelberg 1988

Authors and Affiliations

  • G. Hertting
    • 1
  • S. Wurster
    • 1
  • P. Gebicke-Härter
    • 1
  • C. Allgaier
    • 1
  1. 1.Institute of PharmacologyUniversity of FreiburgFreiburgFederal Republic of Germany

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