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Participation of Regulatory G-Proteins and Protein Kinase C in the Modulation of Transmitter Release in Hippocampus

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Book cover Modulation of Synaptic Transmission and Plasticity in Nervous Systems

Part of the book series: NATO ASI Series ((ASIH,volume 19))

Abstract

1.) Regulatory G-proteins participate in the inhibition of noradrenaline (NA) and acetylcholine (ACh) release following activation of the respective autoreceptors (α2, M2). Isletactivating protein (IAP) and N-ethylmaleimide (NEM) increased the electrically evoked release of [3 H]NA and [3 H]ACh from hippocampal slices and reduced inhibition of release by the autoreceptor agonists clonidine and carbachol as well as its facilitation by the antagonists yohimbine and atropine. In synaptosomes prepared from rabbit hippocampus two polypeptides with molecular weights corresponding to those of Gi α and Go α a were [32P]ADP-ribosylated by IAP in the presence of [3 2P]NAD+. Pretreatment of synaptosomes with NEM reduced the subsequent [3 2P]ADP-ribosylation by IAP in a concentration-dependent manner.

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Authors and Affiliations

  1. Institute of Pharmacology, University of Freiburg, Hermann-Herder-Str. 5, 7800, Freiburg, Federal Republic of Germany

    G. Hertting, S. Wurster, P. Gebicke-Härter & C. Allgaier

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  1. G. Hertting
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  2. S. Wurster
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  3. P. Gebicke-Härter
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  4. C. Allgaier
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Editors and Affiliations

  1. Institut für Pharmakologie, Universität Freiburg, Hermann-Herder-Str. 5, 7800, Freiburg i. Br., Germany

    Georg Hertting

  2. Institut Für Biologie III, Universität Freiburg, Schänzlestr. 1, 7800, Freiburg i. Br., Germany

    Hanns-Christof Spatz

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© 1988 Springer-Verlag Berlin Heidelberg

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Hertting, G., Wurster, S., Gebicke-Härter, P., Allgaier, C. (1988). Participation of Regulatory G-Proteins and Protein Kinase C in the Modulation of Transmitter Release in Hippocampus. In: Hertting, G., Spatz, HC. (eds) Modulation of Synaptic Transmission and Plasticity in Nervous Systems. NATO ASI Series, vol 19. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-73160-0_11

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  • DOI: https://doi.org/10.1007/978-3-642-73160-0_11

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-73162-4

  • Online ISBN: 978-3-642-73160-0

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