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Secretion of Thiophenes by Differentiated Cell Cultures of Tagetes Species

  • Johannes P. F. G. Helsper
  • David H. Ketel
  • Anne C. Hulst
  • Hans Breteler
Part of the NATO ASI Series book series (volume 18)

Abstract

Cell aggregates of T. patulasynthesize and secrete the thiophenes BBT, BBTOH and BBTOAc (see Fig. 1), when cultured in liquid medium. There is a positive correlation between thiophene synthesis and aggregate diameter in the 1 to 12 mm range. No thiophenes are produced by smaller or larger aggregates. Differentiated, hormone-autotrophic “root” cultures, obtained from T. patulatissues after transformation by Agrobacterium tumefaciensLBA 8370, show an extended period of stable BBT and BBTOAc production. The results support the hypothesis that morphological differentiation is required for thiophene biosynthesis. They also show the value of differentiated cell cultures of T. patulafor the production of natural biocides by plant cell biotechnology.

Keywords

Cell Aggregate Aggregate Diameter Aggregate Suspension Related Genus Produce Cork Tissue 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Bohlmann CF, Burkhardt T, Zdero C (1973) Distribution of acetylenes. In: Bohlmann CF, Burkhardt T, Zdero C (eds) Naturally occurring acetylenes. Academic Press, London New York, pp. 340–505Google Scholar
  2. Ketel DH (1987) Callus and cell cultures of Tagetesspecies in relation to production of thiophenes, Thesis Agricultural University, Wageningen, The NetherlandsGoogle Scholar
  3. Sütfeld R (1982) Distribution of thiophene derivatives in different organs of Tagetes patulaseedlings grown under various conditions. Planta 156:536–540CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1988

Authors and Affiliations

  • Johannes P. F. G. Helsper
    • 1
  • David H. Ketel
    • 1
  • Anne C. Hulst
    • 2
  • Hans Breteler
    • 1
  1. 1.NOVAPLANT Cell Biotechnology GroupResearch Institute ItalWageningenThe Netherlands
  2. 2.Department of Food Science, Food and Bioengineering GroupAgricultural UniversityWageningenThe Netherlands

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