Secretion of Thiophenes by Differentiated Cell Cultures of Tagetes Species
Cell aggregates of T. patulasynthesize and secrete the thiophenes BBT, BBTOH and BBTOAc (see Fig. 1), when cultured in liquid medium. There is a positive correlation between thiophene synthesis and aggregate diameter in the 1 to 12 mm range. No thiophenes are produced by smaller or larger aggregates. Differentiated, hormone-autotrophic “root” cultures, obtained from T. patulatissues after transformation by Agrobacterium tumefaciensLBA 8370, show an extended period of stable BBT and BBTOAc production. The results support the hypothesis that morphological differentiation is required for thiophene biosynthesis. They also show the value of differentiated cell cultures of T. patulafor the production of natural biocides by plant cell biotechnology.
KeywordsHigh Performance Liquid Chromatography Acetone Phene Tate Acetylene
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