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Secretion of Thiophenes by Differentiated Cell Cultures of Tagetes Species

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Plant Cell Biotechnology

Part of the book series: NATO ASI Series ((ASIH,volume 18))

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Abstract

Cell aggregates of T. patulasynthesize and secrete the thiophenes BBT, BBTOH and BBTOAc (see Fig. 1), when cultured in liquid medium. There is a positive correlation between thiophene synthesis and aggregate diameter in the 1 to 12 mm range. No thiophenes are produced by smaller or larger aggregates. Differentiated, hormone-autotrophic “root” cultures, obtained from T. patulatissues after transformation by Agrobacterium tumefaciensLBA 8370, show an extended period of stable BBT and BBTOAc production. The results support the hypothesis that morphological differentiation is required for thiophene biosynthesis. They also show the value of differentiated cell cultures of T. patulafor the production of natural biocides by plant cell biotechnology.

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References

  • Bohlmann CF, Burkhardt T, Zdero C (1973) Distribution of acetylenes. In: Bohlmann CF, Burkhardt T, Zdero C (eds) Naturally occurring acetylenes. Academic Press, London New York, pp. 340–505

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  • Ketel DH (1987) Callus and cell cultures of Tagetesspecies in relation to production of thiophenes, Thesis Agricultural University, Wageningen, The Netherlands

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  • Sütfeld R (1982) Distribution of thiophene derivatives in different organs of Tagetes patulaseedlings grown under various conditions. Planta 156:536–540

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Author information

Authors and Affiliations

  1. NOVAPLANT Cell Biotechnology Group, Research Institute Ital, P.O. Box 48, 6700 AA, Wageningen, The Netherlands

    Johannes P. F. G. Helsper, David H. Ketel & Hans Breteler

  2. Department of Food Science, Food and Bioengineering Group, Agricultural University, De Dreijen 12, 6703 BC, Wageningen, The Netherlands

    Anne C. Hulst

Authors
  1. Johannes P. F. G. Helsper
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  2. David H. Ketel
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  3. Anne C. Hulst
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  4. Hans Breteler
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Editor information

Editors and Affiliations

  1. Departamento de Biologia Vegetal, Faculdade de Ciências de Lisboa, R. Escola Politecnica 58, 1294, Lisboa Codex, Portugal

    M. Salomé S. Pais

  2. Chemical Engineering Department, UMIST, P.O. Box 88, Manchester, M60 1QD, UK

    F. Mavituna

  3. Laboratorio de Engenharia Bioquimica, Instituto Superior Tecnico Av. Rovisco Pais, 1000, Lisboa, Portugal

    J. M. Novais

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© 1988 Springer-Verlag Berlin Heidelberg

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Helsper, J.P.F.G., Ketel, D.H., Hulst, A.C., Breteler, H. (1988). Secretion of Thiophenes by Differentiated Cell Cultures of Tagetes Species. In: Pais, M.S.S., Mavituna, F., Novais, J.M. (eds) Plant Cell Biotechnology. NATO ASI Series, vol 18. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-73157-0_41

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  • DOI: https://doi.org/10.1007/978-3-642-73157-0_41

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-73159-4

  • Online ISBN: 978-3-642-73157-0

  • eBook Packages: Springer Book Archive

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