Alkaloids from Organized Atropa belladonna Cultures

  • Jeffrey Lang
  • Robert Hamilton
  • Henrik Pedersen
  • Chee-Kok Chin
Part of the NATO ASI Series book series (volume 18)


Unorganized suspension cultures of some higher plants are known to produce various industrially important products, i.e. nicotine from Nicotiana tobacum [Al-Abta, 1978] and purine alkaloids from Coffea arabica [Frischknecht, 1977, 1980]; however, structurally organized organs or somatic embryos are needed in some cases [Butcher, 1977; Al-Abta, 1978, 1979; Yamada, 1983] for secondary metabolite production. To achieve redifferentiation, media and culture conditions sometimes need to be varied. These changes complicate the selection of optimum production conditions by introducing more manipulative variables. A three stage scheme for production of tropane alkaloids from Atropa belladonna is proposed here.


Somatic Embryo Root Culture Final Root Biomass Secondary Metabolite Production Stage Scheme 
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  1. S. Al-Abta and H.A. Collin, New Phytol., 80, 517 (1978).CrossRefGoogle Scholar
  2. S. Al-Abta, I.J. Galpin, and H.A. Collin, Plant Sci. Lett., 16, 129 (1979).CrossRefGoogle Scholar
  3. D.N. Butcher, Plant Cell, Organ and Tissue Culture (Springer- Verlag, New York, 1977), p. 688.Google Scholar
  4. A.J. Conner and C.P. Meredith, Plant Cell Tiss. Org. Cult., 3, 59 (1984).CrossRefGoogle Scholar
  5. P.M. Frischknecht, T.W. Baumann, and H. Wanner, Planta Med., 31, 344 (1977).CrossRefGoogle Scholar
  6. P.M. Frischknecht and T.W. Baumann, Planta Med., 40, 245 (1980).CrossRefGoogle Scholar
  7. R. Hamilton, H. Pedersen, and C.-K. Chin, in preparation.Google Scholar
  8. B. Kaul and E.J. Staba, Lloydia, 31, 171 (1968).Google Scholar
  9. P. Khanna, G.L. Sharma, and A. Uddin, Ind. J. Exp. Biol., 15, 323 (1977).Google Scholar
  10. R.N. Konar, E. Thomas, and H.E. Street, Ann. Bot., 36, 249 (1972).Google Scholar
  11. T. Murashige and F. Skoog, Physiol. Plant, 15, 473 (1962).CrossRefGoogle Scholar
  12. M. Tabata, H. Yamamoto, N. Hiraoka, Y. Marumoto, and M. Konoshima, J. Phytochem., 10, 723 (1971).CrossRefGoogle Scholar
  13. E. Thomas and M.R. Dewey, From Single Cells to Plants (Wykehen, London, 1985).Google Scholar
  14. P. R. White, The Cultivation of Animal and Plant Cells (Roland, New York, 1954).Google Scholar
  15. H. N. Wood and A. C. Braun, Proc. Nat. Acad. Sci. U.S.A., 47, 1907 (1961).CrossRefGoogle Scholar
  16. Y. Yamada and Y. Fujita, Handbook of Plant Cell Culture (Macmillan, New York, 1983).Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1988

Authors and Affiliations

  • Jeffrey Lang
    • 1
  • Robert Hamilton
    • 1
  • Henrik Pedersen
    • 1
  • Chee-Kok Chin
    • 2
    • 3
  1. 1.Department of Chemical and Biochemical EngineeringRutgers, The State University of New JerseyNew BrunswickUSA
  2. 2.Rutgers, The State University of New JerseyNew BrunswickUSA
  3. 3.Department of Horticulture and ForestryRutgers, The State University of New JerseyNew BrunswickUSA

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