Abstract
The plant-microbe interactions that characterise the Rhizobium-legume symbiosis may be divided somewhat arbitrarily into those concerned with nutritional interactions (biotrophy) and those concerned with nodule development (morphogenesis). At the most intimate stage in the symbiosis, several thousand Rhizobium bacteroids are sustained within each infected plant cell. The host plant maintains a microaerobic oxygen concentration in the vicinity of the bacteroids, sufficient to permit oxidative respiration without denaturation of the highly oxygen-sensitive nitrogen fixation enzyme system. The host plant cells also regulate the supply of respiratory substrates needed by the bacteroids, and assimilate ammonia produced and excreted as a result of N2 fixation. However, before these elaborate physiological interactions can take place, another series of Rhizobium-legume interactions must occur in order to bring about the specific infection of legume roots by Rhizobium and the subseguent differentiation of the highly organised legume nodule structure. As our understanding of these morphogenetic processes unfolds, it will be interesting to compare and contrast these symbiotic interactions with those between a plant and a microbial pathogen. Furthermore, the study of the infection processes for both microbial symbionts and microbial pathogens should help to extend our knowledge and understanding of the normal processes that govern plant cell organisation and morphogenesis.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Anderson, M. A., Sandrin, M. S., and Clarke, A. E. (1984). A high proportion of hybridomas raised to a plant extract secrete antibody to arabinose or galactose. Plant Physiol. 75, 1013–1016
Barclay, S. L. and Smith, A. M. (1986). Rapid isolation of monoclonal antibodies specific for cell surface differentiation antigens. Proc. Natl. Acad. Sci. 83, 4336–4340.
Bradley, D. J., Butcher, G. W., Galfre, G., Wood, E., Brewin, N. J. (1986). Physical association between the peribacteroid membrane and lipopolysaccharide from the bacteroid outer membrane in Rhizobium-infected pea root nodule cells. J. Cell. Sci. 85, 47–61.
Brewin, N. J., Robertson, J. G., Wood, E. A., Wells, B., Larkins, A. P., Galfre, G. and Butcher, G. W. (1985). Monoclonal antibodies to antigens in the peribacteroid membrane from Rhizobium-induced root nodules of pea cross-react with plasma membranes and Golgi bodies. EMBO J. 4. 605–611.
Brewin, N. J., Wood, E. A., Larkins, A. P., Galfre, G., and Butcher G. W. (1986). Analysis of lipopolysaccharide from root nodule bacteroids of Rhizobium leguminosarum using monoclonal antibodies. J. Gen. Microbiol. 132, 1959–1968.
Edge, A.S.B., Faltynek, C.R., Hof, L., Reichert, L. E. and Weber, P. (1981). Deglycosylation of glycoproteins by trifluoromethanesulfonic acid. Anal. Biochem. 118, 131–137.
Finan, T. M., Hirsch, A. M., Leigh, J. A., Johansen, E., Kuldau, G. A., Deegan, S., Walker, G. G., Signer, E. R. (1985). Symbiotic mutants of R. meliloti that uncouple plant from bacterial differentiation. Cell 49, 869–877.
Galfre, C., Milstein, C. (1981). Preparation of monoclonal antibodies: strategies and procedures. Methods Enzymol. 73, 3–46.
Galfre, G. and Butcher, G. W. (1986). Making monoclonal antibodies. In: Immunology in Plant Science, Ed. T. L. Wang, Cambridge University Press.
Goding, J. W. (1987). Monoclonal antibodies: principles and practice. Academic Press (London) (Second Edition).
Golumbeski, G. S. and Dimond, R. L. (1986). The use of tolerisation in the production of monoclonal antibodies against minor determinants. Anal. Biochem. 154, 373–381.
Matthew, W. D. and Paterson, P. H. (1983). The production of a monoclonal antibody that blocks the action of a neurite outgrowth-promoting factor. Cold Spring Harbor Symp. 47, 625–631.
McKearn, T. J., Sarmiento, M. and Weiss, A., Stuart, F. P. and Sitch, F. W. (1978). Selective suppression of reactivity to rat histocompatibility antigens by hybridoma antibodies. Current Topics in Microbiol. and Immunol. 81, 61–65.
Thomas, B., Penn, S. E., Butcher, G. W. and Galfre, G. (1984b). Discrimination between the red- and far-red-absorbing forms of phytochrome from Avena sativa I. by monoclonal antibodies. Planta 160, 382–384.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1988 Springer-Verlag Berlin Heidelberg
About this paper
Cite this paper
Brewin, N.J., Bradley, D.J., Wood, E.A., Kannenberg, E.L., VandenBosch, K.A., Butcher, G.W. (1988). The Use of Monoclonal Antibodies to Investigate Plant-Microbe Interactions in Pea Root Nodules Containing Rhizobium Leguminosarum . In: Scannerini, S., Smith, D., Bonfante-Fasolo, P., Gianinazzi-Pearson, V. (eds) Cell to Cell Signals in Plant, Animal and Microbial Symbiosis. NATO ASI Series, vol 17. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-73154-9_26
Download citation
DOI: https://doi.org/10.1007/978-3-642-73154-9_26
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-73156-3
Online ISBN: 978-3-642-73154-9
eBook Packages: Springer Book Archive