DHE Uptake and PDT Response in an In-Vitro Tumor Model

  • W. P. Jeeves
  • B. C. Wilson
  • P. Spiegl
  • P. Smith
Conference paper
Part of the NATO ASI Series book series (volume 15)

Abstract

Considerable evidence suggests that tumor destruction by Photodynamic Therapy (PDT) results at least in part from primary damage to the tumor vasculature (Henderson and Dougherty, 1984; Star et al, 1986;). Nevertheless, a significant contribution of direct photodynamic tumor cell killing to tumor necrosis cannot be ruled out, the magnitude of which might be expected to be limited by such factors as photosensitizer penetration into tumor tissue, as well as local oxygen concentration. Multicellular tumor spheroids (MTS) are spherical aggregates of cells whose growth is accompanied by the development of heterogeneous subpopulations differing in their proliferation, nutrition, and oxygenation status (Sutherland and Durand, 1976). As such, MTS constitute a useful 3-dimensional tissue culture model intermediate between single cell cultures and poorly-vascularized solid tumors in vivo. We have been using MTS of V-79 Chinese hamster cells in order to study the role of a variety of treatment and biological conditions to determining the response of tumors to the direct photocytotoxic effect of PDT, involving the photosensitizing drug DHE (as Photofrin IITM) in combination with red light.

Keywords

Porphyrin Alan Concession Photofrin Hematoporphyrin 

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References

  1. Henderson BW, Dougherty TJ (1984) Studies on the mechanism of tumor destruction by photoradiation therapy. In: Doiron D, Gomer C (eds) Porphyrin localization and treatment of tumors. Alan R. Liss, New York, p381Google Scholar
  2. Jeeves WP, Wilson BC, Smith P, Ens K, Spiegl P (1987 in press) Growth delay studies of the response of V-79 multicell spheroids exposed to DHE and red light. Photochem PhotobiolGoogle Scholar
  3. Star WM, Marijnissen HPA, van den Berg-Blok Ae, Versteeg JAC, Franken KAP, Reinhold HS (1986). Destruction of rat mammary tumor and normal tissue microcirculation by hematoporphyrin derivative photoradiation observed in vivo in sandwich observation chambers. Cancer Res 46: 2532–2540Google Scholar
  4. Sutherland RM, Durand RE (1976) Radiation response of multicell spheroids - an in vitro tumor model.. Curr Top Radiat Res 11: 87–139Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1988

Authors and Affiliations

  • W. P. Jeeves
    • 1
  • B. C. Wilson
    • 1
  • P. Spiegl
    • 1
  • P. Smith
    • 1
  1. 1.Ontario Cancer Foundation and McMaster UniversityHamiltonCanada

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