Quantitative Aspects of the Interaction of Porphyrins with Cells
Since the efficiency of cell photoinactivation depends both on the intracellular concentration of photosensitizers and on the intrinsic yield of photodynamic processes, comparison of various photosensitizers would be more reliable if these two parameters could be studied independently. In this paper, we describe a new High Performance Liquid Chromatographic (HPLC) method for isocratic separation of dicarboxylic porphyrins and related macrocycles. It is now currently used in our laboratory for the analysis of hematoporphyrin derivative and for control of the purity of newly designed molecules. Analysis of cell extracts by HPLC and spectrophotometry were used to quantify the cellular uptake of a purified porphyrin, hydroxyethylvinyldeuteroporphyrin (HVD). This made possible to correlate the efficiency of cell photoinactivation with the intracellular concentration of the photosensitizer and its localization within the cells.
KeywordsHigh Performance Liquid Chromatographic Ethyl Acetone Leukemia Porphyrin
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