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Quantitative Aspects of the Interaction of Porphyrins with Cells

  • M. Dellinger
  • D. Brault
  • C. Vever-Bizet
Conference paper
Part of the NATO ASI Series book series (volume 15)

Abstract

Since the efficiency of cell photoinactivation depends both on the intracellular concentration of photosensitizers and on the intrinsic yield of photodynamic processes, comparison of various photosensitizers would be more reliable if these two parameters could be studied independently. In this paper, we describe a new High Performance Liquid Chromatographic (HPLC) method for isocratic separation of dicarboxylic porphyrins and related macrocycles. It is now currently used in our laboratory for the analysis of hematoporphyrin derivative and for control of the purity of newly designed molecules. Analysis of cell extracts by HPLC and spectrophotometry were used to quantify the cellular uptake of a purified porphyrin, hydroxyethylvinyldeuteroporphyrin (HVD). This made possible to correlate the efficiency of cell photoinactivation with the intracellular concentration of the photosensitizer and its localization within the cells.

Keywords

High Performance Liquid Chromatographic High Performance Liquid Chromatographic Cellular Uptake High Performance Liquid Chromatographic Method Quantitative Extraction 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Dellinger D, Brault D (1987) Normal-phase high-performance liquid chromatography of free acid dicarboxylic porphyrins and tiematoporphyrin derivative on silica. J Chromatogr, in pressGoogle Scholar
  2. Lipson RL, Baldes J, Olsen AM (1961) The use of a derivative of hematoporphyrin in tumor detection. J Natl Cancer Inst 26: 1–11Google Scholar
  3. Dellinger D, Vever-Bizet C, Brault D, Delgado 0, Rosenfeld C (1986) Cellular uptake of hydroxyethylvinyldeuteroporphyrin (HVD) and photo- inactivation of cultivated human leukemia (Reh6) cells. Photochem Photobiol 43: 639–647CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1988

Authors and Affiliations

  • M. Dellinger
    • 1
    • 2
  • D. Brault
    • 1
    • 2
  • C. Vever-Bizet
    • 1
    • 2
  1. 1.Laboratoire de BiophysiqueINSERM U.201, CNRS UA.481ParisFrance
  2. 2.Muséum National d’Histoire NaturelleParisFrance

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