Photoinactivation of cells studied by 31P-NMR

  • B. Kjeldstad
  • A. Johnsson
  • J. Krane
Conference paper
Part of the NATO ASI Series book series (volume 15)

Abstract

31P-NMR techniques allow the continuous, non-destructive measurements of phosphorous compounds in intact cells. Immediate effects of photodynamic treatment and photosensitization processes can be mapped [2,6]. 31P-NMR also gives information about changes in the pH gradient across membranes and indirectly about ion pumping mechanisms controlling proton fluxes [3]. We have studied the bacterium Propionibacterium acnes, (P. acnes) which produces large amounts of endogenous porphyrins taking part in photosensitization [5]. Externally applied HpD is effective in increasing the photosensitivity [4]. In the present short report we would like to present some new experimental results where 31P-NMR has been used to analyse photodynamic reactions of red light and HpD treatment.

Keywords

NADH Porphyrin Saccharomyces Acne Polyphosphates 

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References

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Copyright information

© Springer- Verlag Berlin Heidelberg 1988

Authors and Affiliations

  • B. Kjeldstad
    • 1
  • A. Johnsson
    • 1
  • J. Krane
    • 1
    • 2
  1. 1.Department of physics, AVHUniversity of TrondheimDragvollNorway
  2. 2.Department of Chemistry, AVHUniversity of TrondheimNorway

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