DNA Damage Caused by Laser Light Activated Hematoporphyrin Derivatives in Isolated Nuclei of Human Melanoma Cells
Hematoporphyrin derivatives (HPDs) are used in the chemotherapy of various tumors. They are a mixture of dimers and oligomers of hematoporphyrin linked by ether and ester bonds (for reviews see Kessel 1986a; Pottier and Truscott 1986). They accumulate in tumor cells and are cytotoxic after photosensitization with laser light. Reactive oxygen species formed during irradiation of HPDs are most likely responsible for the cytotoxicity observed (Keene et al. 1986; van Steveninck et al. 1986; Buettner and Hall 1987). However, the cellular target for these reactive oxygen species has not yet been identified. Critical oxidizable sites of mitochondria or the outer cellular membrane have been related to laser-activated HPD toxicity (Atlante et al. 1986; Kessel 1986b; Moan and Vistnes 1986; Salet 1986).
KeywordsToxicity Hydrogen Peroxide Hydroxyl Agarose Bromide
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