Advertisement

Organic Solvents Affect Rat Synaptosome Membrane Acetylcholinesterase and Adenosine Triphosphatase In Vitro

  • M. Korpela
Part of the Archives of Toxicology book series (TOXICOLOGY, volume 12)

Abstract

The common toxic effect of organic solvents is their CNS depression. The mechanism of this anesthetic action is a matter of controversy, but recent investigations suggest that anesthetic agents can act nonspecifically with large hydrophobic regions of membrane proteins rather than with membrane bilayer. Changes in the lipid composition of nerve cell membrane have not been shown to happen at clinical concentrations of anesthetic agents (Franks and Lieb 1982).

Keywords

ATPase Activity AChE Activity Nerve Cell Membrane Adenosine Triphosphatase Anesthetic Action 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Ellman GL, Courtney KD, Andres A Jr, Featherstone RM (1961) A new and rapid colorimetric determination of acetylcholine-esterase activity. Biochem Pharmacol 7:88–95PubMedCrossRefGoogle Scholar
  2. Franks NP, Lieb WR (1982) Molecular mechanisms of general anaesthesia. Nature 300:487–493PubMedCrossRefGoogle Scholar
  3. Greenberg M, Tsong TY (1984) Detergent solubilization and affinity purification of a local anesthetic binding protein from mammalian axonal membranes. J Biol Chem 259:13241–13245PubMedGoogle Scholar
  4. Holmberg B, Jakobson I, Malmfors T (1974) The effect of organic solvents on erythrocytes during hypotonic hemolysis. Environ Res 7:193–205CrossRefGoogle Scholar
  5. James RC (1985) The toxic effects of organic solvents. In: Williams PL, Burson JL (eds) Industrial toxicology. Safety and health applications in the workplace. Van Nostrand Reinhold, New York, pp 230–259Google Scholar
  6. Korpela M, Tähti H (1986) The effect of selected organic solvents on intact human red cell membrane acetylcholinesterase in vitro. Toxicol Appl Pharmacol 85:257–262PubMedCrossRefGoogle Scholar
  7. Lowry OH, Lopez JA (1946) The determination of inorganic phosphate in the presence of labile phosphate esters. J Biol Chem 162:421–428PubMedGoogle Scholar
  8. Nagy A, Delgado-Escuesta AV (1984) Rapid preparation of synaptosomes from mammalian brain using nontoxic iso-osmotic gradient material (Percoll). J Neurochem 43:1114–1123PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag 1988

Authors and Affiliations

  • M. Korpela
    • 1
  1. 1.Department of Biomedical SciencesUniversity of TampereTampereFinland

Personalised recommendations