Organization and Expression of the Nuclear Genome
During the last decade enormous progress was made in the investigation of the structure of the ciliate genome. Whereas for a long time ciliates have been thought to be rather burlesque relicts of evolution of interest only as an ecological niche for a few enthusiasts, they are now becoming more and more important for the research on basic mechanisms of the molecular biology of eukaryotic genes. Most of this progress was made on a few genera of ciliates: among the hypotrichs Oxytricha, Stylonychia and Euplotes and among the holotrichs essentially on Tetrahymena (see Gall 1984). Paramecium, most important in the past, seems to be somewhat ousted from the centre of interest in this field of research. Nevertheless, as it was demonstrated very recently by the discovery of its deviation in the usage of the “universal” genetic code, Paramecium and its molecular genetics remain most important for our understanding of the organization and evolution of eukaryotic genes.
KeywordsCellulose Codon Immobilization Glutamine Gall
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- Blackburn EH (1982) Characterization and species differences of rDNA: protozoans. In: Busch H, Rothblum L (eds) The cell nucleus, vol 10. Academic Press, London New York, pp 145–170Google Scholar
- Boveri Th (1899) Die Entwicklung von Ascaris megalocephala mit besonderer Rücksicht auf die Kernverhältnisse. Kupfer-Festschrift. Fischer, JenaGoogle Scholar
- Cummings DJ (1977) Methods for the isolation of nuclei from ciliated protozoans. In: Stein G, Stein J, Kleinsmith L (eds) Methods in cell physiology, vol 16. Academic Press, London New York, pp 97–112Google Scholar
- Cummings DJ, Tait A (1975) The isolation of nuclei from Paramecium aurelia. In: Prescott DM (ed) Methods in cell biology, vol 9. Academic Press, London New York, pp 281–309Google Scholar
- Nanney DL (1980) Experimental ciliatology. An introduction to genetic and developmental analysis in ciliates. Wiley & Sons, New YorkGoogle Scholar
- Preer JR, Jr, Preer LB (1979) The size of macronuclear DNA and its relationship to models for maintaining genic balance. J Protozool 26:14–18Google Scholar
- Raikov IB (1982) The protozoan nucleus. Morphology and evolution. Springer, Berlin Heidelberg New YorkGoogle Scholar
- Schwartz V, Meister H (1975) Die Extinktion der feulgengefärbten Makronucleusanlage von Paramecium bursaria in der DNS-armen Phase. Arch Protistenk 117:60–64Google Scholar
- Shiomi Y, Higashinakagawa T, Saiga H, Mita T (1980) Metrizamide isopycnic centrifugation for the isolation of macro- and micronuclei from Paramecium. J UOEH 2:323–330Google Scholar
- Soldo AT, Brickson SA, Larin F (1981) The kinetic and analytical complexities of the DNA genomes of certain marine and fresh-water ciliates. J Protozool 28:377–383Google Scholar
- Sonneborn TM (1974) Paramecium aurelia. In: King RC (ed) Handbook of genetics, vol 2. Plenum, New York, pp 469–594Google Scholar
- Steinbrück G (1986) Molecular reorganization during nuclear differentiation in ciliates. In: Hennig W (ed) Results and problems in cell differentiation, vol 13. Springer, Berlin Heidelberg New York Tokyo, pp 105–174Google Scholar