Summary
In order to facilitate the identification and manipulation of plasmids in Azospirillum we attempted to mark them by insertion of the kananycin-resistant transposon TnS. Plasmids pACYC184::Tn5-Mob and pBR322::Tn5 were used to introduce Tn5 into A. brasilense Sp7. A high efficiency of transfer was obtained with pACYC184 containing the Mob region of RP4. All the Sp7 kmr transconjugants isolated were the result of Tn5 transposition to the Azospirillum genome. Four per cent of these contained the Tn5-Mob transposon on a replicon mobilizable and stable in E. coli. Using a Sp7::Tn5Mob strain containing the Tn5-Mob on a mobilizable replicon, we tested the mobilization of A. brasilense plasmids to other genera. We were able to find kmr transconjugants only with A. tumefaciens.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Elmerich, C. (1984). Biotechnology 2, 967–978
Franche, C. and Elmerich, C. (1981). Ann. Microbiol.(Inst. Pasteur) 132A, 3–17
Rosenberg, C., Boistard, P., Dénarié, J. and Casse-Delbart, F. (1981). Mol. Gen. Genet. 184, 326–333
Currier, W.B., Gordon, M.P., Chilton, M.D. and Nester, E.W. (1975). J. Bacteriol. 123, 255–264
Comai, L. and Kosuge, T. (1980). J. Bacteriol. 143, 950–957
Banfalvi, Z., Sakanyan, V., Kancz, C., Kiss, A., Dusha, I. and Kondorosi, A. (1981). Mol. Gen. Genet. 184,318–325
Rosenberg, C., Boistard, P., Dénarié, J. and Casse-Delbart, F. (1981). Mol. Gen. Genet. 184, 326–333
Plazinsky, J., Dart, P.J. and Rolfe, B.G. (1983). J. Bacterio1. 155, 1429–33
Simon, R., Priefer, U. and Pühler, A. (1984). Biotechnology 1, 784–791
Boyer, H.W. and Roulland-Dussoix, D. (1969). J. Mol. Biol. 41, 459
Hanahan, D. (1983). J. Mol. Biol. 166, 577
Van Larebeke, N., Genetello, C., Schell, J., Schilperrort, R.A., Hermans, A.K., Hernalsteens, J.P. and Van Montagu, M. (1975). Nature 255, 742–743
Hooykaas, P.J.J., Snijdewint, F.G.M. and Schilperoort, R.A. (1982). Plasmid 8, 73–82
Simon, R. (1984). Mol. Gen. Genet. 196, 413–420
Ditta, G., Stanfield, S., Corbin, D. and Helinski, D. (1980). Proc. Natl. Acad. Sci. USA 77, 7347–7351
Bozouklian, H., Fogher, C. and Elmerich, C. (1986). Ann. Microbial. (Inst. Pasteur) 137B, 3–18
Gauthier, D. and Elmerich, C. (1977). FEMS Microbiol. Letters, 2, 101–104
Vincent, J.M. (1970). Int. Biology Progr. Hand-book n° 15. Blackwell Pub. Co., Oxford.
Eckhardt, T. (1978). Plasmid 1, 584–588
Casse, F., Boucher, C., Julliot, J.S., Michel,M. and Dénarié, J. (1979). J. Gen. Microbiol. 113, 229–242
Maniatis, T., Fritsch, E.F. and Sambrook, J. (1982). In “Molecular Cloning”. Cold Spring Harbor, New York.
Southern, E.M. (1975). J. Mol. Biol. 98, 503–517
Simon, R., Priefer, U. and Pühler, A. (1983). Biotechnology 2, 184–190
Fogher, C., Dusha, I., Barbot, P. and Elmerich, C. (1985). FEMS Microb. Letters 30, 245–249
Franche, C., Canélo, E., Gauthier, D. and Elmerich, C. (1981). FEMS Microbiol. Letters 10, 199–202
de Bruijn, F.J. and Lupski, J.R. (1984). Gene 27, 131–149
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1988 Springer-Verlag Berlin Heidelberg
About this paper
Cite this paper
Pampaluna, M., Antonelli, M.N., Piana, L., Fogher, C. (1988). Mobilization of Cryptic Plasmids in Azospirillum. In: Klingmüller, W. (eds) Azospirillum IV. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-73072-6_8
Download citation
DOI: https://doi.org/10.1007/978-3-642-73072-6_8
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-73074-0
Online ISBN: 978-3-642-73072-6
eBook Packages: Springer Book Archive