Tn5-Mutagenesis in Azospirillum Brasilense

  • M. Vanstockem
  • A. Milcamps
  • K. Michiels
  • J. Vanderleyden
  • A. P. Van Gool
Conference paper


A system is described for transposon mutagenesis of Azospirillum brasilense using the mobilizable plasmid pSUP2021. Mutants were selected on rich medium containing kanamycin and streptomycin. All the auxotrophs isolated were impaired in their sulfur metabolism. Calcofluor-dark Tn5 mutants were tested for their ability to form flocs.


Azospirillum brasilense Tn5 mutagenesis auxotrophy Cal mutants 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Elmerich, C. and Franche, C. (1982). In W. Klingmüller (ed.). Azospirillum: Genetics, Physiology, Ecology. Exs 42, Birkhaüser, BaselGoogle Scholar
  2. 2.
    Singh, M. and Klingmüller D.G. (1986). Mol. Gen. Genet. 202, 136–142CrossRefGoogle Scholar
  3. 3.
    Vanstockem, M., Michiels, K., Vanderleyden, J. and Van Gool, A. (1987). Appl. Env. Microbiol. 53, 410–415Google Scholar
  4. 4.
    Selvaraj, G. and Iyer, V.N. (1983). J. Bacteriol. 156, 1292–1300PubMedGoogle Scholar
  5. 5.
    Simon, R., Priefer, U. and Pühler, A. (1983). Bio/Technology 1, 784–790CrossRefGoogle Scholar
  6. 6.
    Baldani, V.L.D., Alvarez, M., Baldani, J.I. and Döbereiner, J. (1986). Plant and Soil 90, 35–46CrossRefGoogle Scholar
  7. 7.
    Michiels, K., Maris, M., Vanstockem, M., Vanderleyden, J. and Van Gool, A. (1985). Arch. Intern. Physiol. Biochim. B153Google Scholar
  8. 8.
    Ditta, G., Stanfield, S., Corbin, D. and Helinski, D. (1980). Proc. Natl. Acad. Sci.. USA 77, 7347–7351PubMedCrossRefGoogle Scholar
  9. 9.
    Figurski, D. and Helinski, D. (1979). Proc. Natl. Acad. Sci. USA 76, 1648–1652PubMedCrossRefGoogle Scholar
  10. 10.
    Maniatis, T., Fritsch, E. and Sambrook, J. (1982). Molecular cloning, a laboratory manual. Cold Spring Harbor Laboratory, N.Y.Google Scholar
  11. 11.
    Kado, C.I. and Liu, S. (1981). J. Bacteriol. 145, 1365–1373PubMedGoogle Scholar
  12. 12.
    Silhavy, T., Berman, M. and Enquist, L. (1984). Experiments with gene fusions. Cold Spring Harbor Laboratory, N.Y.Google Scholar
  13. 13.
    Davis, R., Botstein, B. and Roth, J. (1980). A manual for genetic engineering. Advanced bacterial genetics. Cold Spring Harbor, N.Y.Google Scholar
  14. 14.
    Sadasivan, L. and Neyra, C. (1985). J. Bacteriol. 1631, 716–723Google Scholar
  15. 15.
    Meade, H., Long, S., Ruvkun, G., Brown, S., Ausubel, F. (1982). J. Bacteriol. 149, 114–122PubMedGoogle Scholar
  16. 16.
    Hughes, J. and McCully, N.E. (1975). Stain Technol. 50, 319–329PubMedGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1988

Authors and Affiliations

  • M. Vanstockem
    • 1
  • A. Milcamps
    • 1
  • K. Michiels
    • 1
  • J. Vanderleyden
    • 1
  • A. P. Van Gool
    • 1
  1. 1.F.A. Janssens Memorial Laboratory for GeneticsCatholic University of LeuvenHeverleeBelgium

Personalised recommendations