Tn5-Mutagenesis in Azospirillum Brasilense

  • M. Vanstockem
  • A. Milcamps
  • K. Michiels
  • J. Vanderleyden
  • A. P. Van Gool
Conference paper

Summary

A system is described for transposon mutagenesis of Azospirillum brasilense using the mobilizable plasmid pSUP2021. Mutants were selected on rich medium containing kanamycin and streptomycin. All the auxotrophs isolated were impaired in their sulfur metabolism. Calcofluor-dark Tn5 mutants were tested for their ability to form flocs.

Keywords

Azospirillum brasilense Tn5 mutagenesis auxotrophy Cal mutants 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1988

Authors and Affiliations

  • M. Vanstockem
    • 1
  • A. Milcamps
    • 1
  • K. Michiels
    • 1
  • J. Vanderleyden
    • 1
  • A. P. Van Gool
    • 1
  1. 1.F.A. Janssens Memorial Laboratory for GeneticsCatholic University of LeuvenHeverleeBelgium

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