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A Tn5 Induced NifA Like Mutant of Azospirillum Brasilense

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Azospirillum IV

Summary

Using the suicide vector pGS9, transposon Tn5 mutagensis was employed to isolate three Nif−-mutants of A. brasilense strain ATCC29710 (Cd). One of the mutants, Nif27, has been characterized physically as well as biochemically. This mutant is not defective in nifHDK genes. The cloned nif27::Tn5 DNA does not show homology to the nif/qln gene probes of K. pneumoniae or to the fix gene probe of Bradyrhizobium japonicum. The results of western blotting show that Nif27 is unable to synthesize the nitrogenase iron protein and thus this mutant is defective in a nifA like gene.

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Author information

Authors and Affiliations

  1. Max-Planck-Institut für Züchtungsforschung, D-5000, Köln 30, Germany

    M. Singh

  2. Lehrstuhl für Genetik, Universität Bayreuth, D-8580, Bayreuth, Germany

    W. Klingmüller

Authors
  1. M. Singh
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  2. W. Klingmüller
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Editor information

Editors and Affiliations

  1. Lehrstuhl für Genetik, Universität Bayreuth, Universitätsstraße 30, 8580, Bayreuth, Germany

    Walter Klingmüller

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© 1988 Springer-Verlag Berlin Heidelberg

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Singh, M., Klingmüller, W. (1988). A Tn5 Induced NifA Like Mutant of Azospirillum Brasilense . In: Klingmüller, W. (eds) Azospirillum IV. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-73072-6_4

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  • DOI: https://doi.org/10.1007/978-3-642-73072-6_4

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-73074-0

  • Online ISBN: 978-3-642-73072-6

  • eBook Packages: Springer Book Archive

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