Skip to main content
SpringerLink
Log in

Cloning of Azospirillum Brasilense Sequences Promoting Transcription in Escherichia Coli

  • Conference paper
Azospirillum IV

Summary

Some genes of A.brasilense can be expressed in E.coli. We take advantage of this property for the isolation of A.brasilense promoter sequences. DNA fragment of A.brasilense SP6 were cloned into the BamHI site of plasmid pKK232–8 upstream the chloramphenicol resistance (CAT) gene which lacks its own promoter. Some of the Azospirillum DNA sequences selected for the ability to promote CAT gene transcription have been further characterized either for the level of resistance to chloramphenicol and for sequence analysis.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 84.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Rosenberg,M. and Court D. (1979). Ann.Rev.Genet. 13; 319–353

    Article  PubMed  CAS  Google Scholar 

  2. Gussing, G.N., Ronson, C.W. and Ausubel F.M. (1986). Ann.Rev.Genet. 20; 567–592

    Article  Google Scholar 

  3. Dixon R.A. (1984). J.Gen.Microbiol. 130; 2745–2755

    PubMed  CAS  Google Scholar 

  4. Klingmuller W. (ed) (1985). Azospirillum III: Genetics, Physiology, Ecology. Springer-Verlag, Heidelberg.

    Google Scholar 

  5. Bani,D., Barberio, C., Bazzicalupo, M., Favilli, F., Gallori,E. and Polsinelli M. (1980). J.Gen.Microbiol. 119; 239–244

    CAS  Google Scholar 

  6. Gauthier, D. and Elmerich C. (1977). FEMS Microbiol.Lett. 2; 101–104

    Article  CAS  Google Scholar 

  7. Bazzicalupo, M., Fani, R., Gallori, E., Turbanti, L. and Polsinelli M. (1987). Mol. Gen. Genet. 206; 76–80

    Article  CAS  Google Scholar 

  8. Brosius J. (1984). Gene 27; 151–170

    Article  PubMed  CAS  Google Scholar 

  9. Hanahan D. (1983). J.Mol.Biol. 145; 713–734

    Google Scholar 

  10. Shaw W.V. (1975). Methods in Enzymology, Academic Press, N.Y. 43; 740–746

    Google Scholar 

  11. Dente, L., Sollazzo, M., Baldari, C., Cesareni, G. and Cortese R. (1985). InGlover D.M. ed. DNA cloning: a practical approach, IRL Press, Oxford-Washington, vol I; 101–107

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Dipartimento di Biologia Animale e Genetica, Università di Firenze, Via Romana 17, 50125, Firenze, Italy

    Concetta Schipani, Marco Bazzicalupo, Enzo Gallori, Ettore Luzi, Luca Turbanti & Renato Fani

Authors
  1. Concetta Schipani
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Marco Bazzicalupo
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Enzo Gallori
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Ettore Luzi
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Luca Turbanti
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Renato Fani
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Lehrstuhl für Genetik, Universität Bayreuth, Universitätsstraße 30, 8580, Bayreuth, Germany

    Walter Klingmüller

Rights and permissions

Reprints and permissions

Copyright information

© 1988 Springer-Verlag Berlin Heidelberg

About this paper

Cite this paper

Schipani, C., Bazzicalupo, M., Gallori, E., Luzi, E., Turbanti, L., Fani, R. (1988). Cloning of Azospirillum Brasilense Sequences Promoting Transcription in Escherichia Coli. In: Klingmüller, W. (eds) Azospirillum IV. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-73072-6_2

Download citation

  • DOI: https://doi.org/10.1007/978-3-642-73072-6_2

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-73074-0

  • Online ISBN: 978-3-642-73072-6

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation