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Advances in Genetics of Azospirillum Brasilense Sp7: Use of Tn5 Mutagenesis for Gene Mapping and Identification

  • Conference paper
Azospirillum IV

Summary

Tn5 site-directed mutagenesis was used to identify nif genes and to localize plasmid genes from A. brasilense Sp7 (ATCC29145). A DNA region covering 30 kb and containing the nitrogenase structural genes (nifHDK) has been cloned. The presence of nif genes in the 20 kb located next to nifHDK was explored by Tn5 mutagenesis and two new nif loci were identified. Homology between Rhizobium meliloti nodulation genes (nod and hsn) and Azospirillum total DNA was previously reported. A 10 kb EcoRI fragment homologous to hsn was cloned in pUC18, to yield pAB502. Preliminary experiments suggested that the hsn homologous region was located on the 90 Md plasmid contained in strain Sp7. This was confirmed after mutagenesis of pAB502 by Tn5-mob and recombination of the insertions in the 90 Md plasmid.

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Authors and Affiliations

  1. Département des Biotechnologies, Institut Pasteur, Unité de Physiologie Cellulaire, 28 rue du Docteur Roux, 75724, Paris Cedex 15, France

    M. Galimand, C. Vieille, B. Perroud, I. Onyeocha & C. Elmerich

Authors
  1. M. Galimand
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  2. C. Vieille
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  3. B. Perroud
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  4. I. Onyeocha
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  5. C. Elmerich
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Editor information

Editors and Affiliations

  1. Lehrstuhl für Genetik, Universität Bayreuth, Universitätsstraße 30, 8580, Bayreuth, Germany

    Walter Klingmüller

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© 1988 Springer-Verlag Berlin Heidelberg

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Galimand, M., Vieille, C., Perroud, B., Onyeocha, I., Elmerich, C. (1988). Advances in Genetics of Azospirillum Brasilense Sp7: Use of Tn5 Mutagenesis for Gene Mapping and Identification. In: Klingmüller, W. (eds) Azospirillum IV. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-73072-6_1

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  • DOI: https://doi.org/10.1007/978-3-642-73072-6_1

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-73074-0

  • Online ISBN: 978-3-642-73072-6

  • eBook Packages: Springer Book Archive

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