Skip to main content

Hypoxis spp.: Micropropagation and In Vitro Production of Hypoxoside

  • Chapter
Medicinal and Aromatic Plants I

Part of the book series: Biotechnology in Agriculture and Forestry ((AGRICULTURE,volume 4))

Abstract

Species of the genus Hypoxis L. are used for herbal remedies throughout the world. In Asia use is made of H. aurea (Lewis and Elvin-Lewis 1977), while in the West Indies H. decumbens and H. scuronera are collected for curing tumors of the testicles (Hartwell 1967). Utilization of this genus is, however, most frequent on the African continent, where species such as H. rooperi are extensively employed in folk medicine (Watt and Breyer-Brandwijk 1962). The merit of these folk medicines has been recognized by the scientific, medical, and commercial communities. This has resulted in attempts to isolate the medicinally active agents, to determine their range of application, to propagate the plants, and to produce the required chemicals by suspension culture.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 84.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Amorim HV, Dougall DK, Sharp WR (1977) The effect of carbohydrate and nitrogen concentration on phenol synthesis in Paul’s Scarlet Rose cells grown in tissue culture. Physiol Plant 39:91–95.

    Article  CAS  Google Scholar 

  • Barclay AS, Perdue RE (1976) Distribution of anticancer activity in higher plants. Cancer Treat Rep 60:181–1113.

    Google Scholar 

  • Bews JW, Vanderplank JE (1930) Storage and other carbohydrates in a Natal succulent and a Natal geophyte and their behaviour before, during and after the winter resting season. Ann Bot (London) 44:689–719.

    CAS  Google Scholar 

  • Bryant AT (1966) Zulu medicine and medicine-man. Struik, Cape Town.

    Google Scholar 

  • Butcher DN (1977) Secondary products in tissue cultures. In: Reinert J, Bajaj YPS (eds) Applied and fundamental aspects of plant cell, tissue, and organ culture. Springer, Berlin Heidelberg New York, pp 668–693.

    Google Scholar 

  • Constabel F (1968) Gerbstoffproduktion der Calluskulturen von Juniperis communis L. Planta 79:58–64.

    Article  CAS  Google Scholar 

  • Dahlgren RMT, Clifford HT (1982) The monocotyledons: A comprehensive study. Academic Press, London New York.

    Google Scholar 

  • Dougall DK (1979) Factors affecting the yields of secondary products in plant tissue cultures. In: Sharp WR, Larsen PO, Paddock EF, Raghavan V (eds) Plant cell and tissue culture — Principles and Applications. Ohio State Univ Press, Columbus, pp 727–743.

    Google Scholar 

  • Drewes SE, Hall AJ, Learmonth RA, Upfold U (1984) Isolation of hypoxoside from Hypoxis rooperi and the synthesis of (E)-1,5-bis(3′,4′-dimethyloxyphenyl)pent-4-en-1-yne. Phytochemistry 23:1313–1316.

    Article  CAS  Google Scholar 

  • Dyer RA (1976) The genera of South African flowering plants. 2: Gymnosperms and monocotyledons. Dep Agric Tech Serv, Pretoria.

    Google Scholar 

  • Fernandes A, Neves JB (1961) Sur la caryologie de quelques monocotylédones africaines. R IVe Reun AETFAT, pp 439-464.

    Google Scholar 

  • Forrest GI (1969) Effects of light and darkness on polyphenol distribution in the tea plant (Camellia sinensis L.). Biochem J 113:773–781.

    PubMed  CAS  Google Scholar 

  • Hahlbrock K (1972) Isolation of apigenin from illuminated cell suspension cultures of soybean, Glycine max. Phytochemistry 11:165–167.

    Article  CAS  Google Scholar 

  • Harborne JB (1980) Plant phenolics. In: Bell EA, Charlwood BV (eds) Secondary plant products. Springer, Berlin Heidelberg New York, pp 329–402.

    Chapter  Google Scholar 

  • Hartwell JL (1967) Plants used against cancer. A survey. Lloydia 30:379–436.

    Google Scholar 

  • Hegenauer R (1963) Chemotaxonomie der Pflanzen, Bd 2. Birkhäuser, Basel, pp 234–236.

    Google Scholar 

  • Heideman ME (1979) Taxonomic studies in the genus Hypoxis L. (Hypoxidaceae) on the Witwaters-rand. MSc Thesis, Univ Witwatersrand, Johannesburg.

    Google Scholar 

  • Hussey G (1976) Plantlet regeneration from callus and parent tissue in Ornithogalum thysoides. J Exp Bot 27:375–382.

    Article  CAS  Google Scholar 

  • Kapoor LD, Kapoor SL, Srivastava SN, Singh A, Sharma PC (1971) Survey of Indian plants for saponins, alkaloids and flavonoids. II. Lloydia 34:94–102.

    PubMed  CAS  Google Scholar 

  • Kreuzaler F, Hahlbrock K (1973) Flavonoid glycosides from illuminated cell suspension cultures of Petroselinum hortense. Phytochemistry 12:1149–1153.

    Article  CAS  Google Scholar 

  • Kurz WGW, Constabel F (1979) Plant cell cultures, a potential source of pharmaceuticals. Adv Appl Microbiol 25:209–239.

    Article  PubMed  CAS  Google Scholar 

  • Lewis WH, Elvin-Lewis M (1977) Medical botany. Plants affecting man’s health. John Wiley & Sons, New York, p 375.

    Google Scholar 

  • Marini-Bettolo GB, Patamia M, Nicoletti M, Galeffi C, Messana I (1982) Research on African medicinal plants. III. Hypoxoside a new glucoside of uncommon structure from Hypoxis obtusa Buch. Tetrahedron 11:1683–1687.

    Article  Google Scholar 

  • Marini-Bettolo GB, Nicoletti M, Messana I, Galeffi C, Msonthi JD, Chapya WA (1985) Research on African medicinal plants X. Glucosides of Hypoxis nyasica Bak. The structure of nyasoside, a new glucoside biologically related to hypoxoside. Tetrahedron 41:665–670.

    Article  CAS  Google Scholar 

  • Markotter EI (1936) Die lewensgeskiedens van sekere geslagte van die Amaryllidaceae. Ann Univ Stellenbosch XIV, A2.

    Google Scholar 

  • McClure JW (1979) The physiology of phenolic compounds in plants. In: Swain T, Harborne JB, Sumere CF van (eds) Recent advances in phytochemistry: Biochemistry of plant phenolics, vol 12. Plenum, New York, pp 525–556.

    Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant 15:473–497.

    Article  CAS  Google Scholar 

  • Page YM (1984) The regeneration of Hypoxis rooperi S. Moore and production of hypoxoside in vitro. Ph D Thesis, Univ Natal, Pietermaritzburg.

    Google Scholar 

  • Page YM, Staden J van (1984) In vitro propagation of Hypoxis rooperi. Plant Cell Tiss Org Cult 3:359–362.

    Article  CAS  Google Scholar 

  • Page YM, Staden J van (1986) In vitro propagation of Hypoxis rooperi from flower buds. S Afr J Bot 52:261–264.

    Google Scholar 

  • Page YM, Staden J van (1987) In vitro production of hypoxoside. Plant Cell Tiss Org Cult 9:131–136.

    Article  CAS  Google Scholar 

  • Pegel KH (1973a) Extraction of sterolins from plant material. S Afr Patent J 7201855.

    Google Scholar 

  • Pegel KH (1973b) Extraction of phytosterol glucosides from Hypoxis tubers. German Offenlegungsschrift 2312285.

    Google Scholar 

  • Pegel KH (1975) Extraction of sterolins from plant material. UK Patent Specif 1417272.

    Google Scholar 

  • Pegel KH (1979) Active plant extracts of the Hypoxidaceae and their use. U S Patent 4160860.

    Google Scholar 

  • Pegel KH (1980) Active plant extracts of the Hypoxidaceae and their use. U S Patent 4198401.

    Google Scholar 

  • Pierik RLM, Steegmans HHM (1975) Freesia plantlets from flower buds cultivated in vitro. Neth J Agric Sci 23:334–337.

    Google Scholar 

  • Reinert J, Clauss H, Ardenne R von (1964) Anthocyanbildung in Gewebekulturen von Haplopappus gracilis in Licht verschiedener Qualität. Naturwissenschaften 51:87.

    Article  CAS  Google Scholar 

  • Reisch J, Mollman H (1974) Wirkstoffextrakt aus Hypoxis species und fehafren zu seiner Gewinnung. German Offenlegungsschrift 2251695.

    Google Scholar 

  • Skryzypczakowa L (1970) Flavonoidy 1 biosyntetycznie pokrewne zwiaski jako czynniki taksonomiczne klasy jednolisciennych. Wiadom Bot 14:55–63.

    Google Scholar 

  • Stickland RG, Sunderland N (1972) Photo-control of growth and of anthocyanin and chlorogenic acid production in cultured callus tissue of Happlopappus gracilis. Ann Bot (London) 36:671–685.

    CAS  Google Scholar 

  • Van Staden J (1981) Inhaltsstoffe der Hypoxis rooperi einer wertvollen Arzneipflanze des südlichen Afrikas. Dtsch Apotheker 7:1–7.

    Google Scholar 

  • Van Staden J, Page YM, Forsyth C (1986) Antagonistic effect of hypoxoside on kinetin-induced growth of soybean callus. S Afr J Bot 52:408–412.

    Google Scholar 

  • Walford SN (1979) Hypoxis rooperi (Hypoxidaceae), eine bisher in Europa wenig bekannte Heilpflanze Afrikas. Dtsch Apotheker 11:642.

    Google Scholar 

  • Wall ME, Krider MM, Krewson CF, Eddy CR, Willaman JJ (1954) Steroidal sapogenins, XII. Supplementary table data for steroidal sagpogenins VII. U S Dep Agric.

    Google Scholar 

  • Warren KS (1972) Medicinal substances. UK Patent Specif 1259503.

    Google Scholar 

  • Watt JM, Breyer-Brandwijk MG (1962) The medicinal and poisonous plants of Southern and Eastern Africa, 2nd edn. Livingston, Edinburgh.

    Google Scholar 

  • Westcott RJ, Henshaw GG (1976) Phenolic synthesis and phenylalamne-ammonia-lyase activity in suspension cultures of Acer pseudoplatanus L. Planta 131:67–73.

    Article  CAS  Google Scholar 

  • Wilsenach R (1967) Cytological observations of Hypoxis: I. Somatic chromosomes and meiosis in some Hypoxis species. J S A Bot 33:75–84.

    Google Scholar 

  • Wilsenach R, Papenfus JN (1967) Cytological observations of Hypoxis. II. Pollen germination, pollen tube growth and haploid chromosome numbers in some Hypoxis species. J S A Bot 33:111–116.

    Google Scholar 

  • Wilsenach R, Warren JL (1967) Cytological observations of Hypoxis: III. Embryo sac development in Hypoxis rooperi and H. filiformis. J S A Bot 33:133–140.

    Google Scholar 

  • Wood SE (1976) A contribution to the knowledge of the genus Hypoxis L. (Hypoxidaceae) in Natal, South Africa. M Sci Thesis, Univ Natal, Pietermaritzburg.

    Google Scholar 

  • Zenk MH, El-Shagi H, Ulbrich B (1977) Production of rosmarinic acid by cell suspension cultures of Coleus blumei. Naturwissenschaften 64:585–586.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 1988 Springer-Verlag Berlin Heidelberg

About this chapter

Cite this chapter

van Staden, J., Bayley, A.D. (1988). Hypoxis spp.: Micropropagation and In Vitro Production of Hypoxoside. In: Bajaj, Y.P.S. (eds) Medicinal and Aromatic Plants I. Biotechnology in Agriculture and Forestry, vol 4. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-73026-9_23

Download citation

  • DOI: https://doi.org/10.1007/978-3-642-73026-9_23

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-73028-3

  • Online ISBN: 978-3-642-73026-9

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics